Patent US2665869A successfully refreshed from Google Patents.
EP0452894A2
Expired / Lapsed
EP
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Human cells for the high expression of genes inserted in episomal recombinant DNA, their preparation and their use
Assignee: Consiglio Nazionale delle Richerche
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Filing: 1991-04-17
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Granted: —
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Expires: 2011-04-17
Abstract
Abstract Modified human cells are described which allow the expression of products encoded by any gene under the control of HIV-1 LTR. <IMAGE>
Inventors
- 👤 Massimo Negrini
- 👤 Paola Rimessi
- 👤 Roberto Manservigi
- 👤 Giuseppe Barbanti-Brodano
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 02:16
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/87; C12N15/90; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122; C12N2800/00; C12N2800/10; C12N2800/108
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
37 words
Human 293 cells the cellular genome of which was modified with pSU3tat and pHEBo integration vectors and which were successively transformed with episomal vectors pRPneoU3RX, where X represents a gene under the control of the HIV-1 LTR.
Claim 2
Dependent Claim
12 words
Cells according to claim 1, in which the episomal vector is pRPneoU3RCAT.
Claim 3
Dependent Claim
11 words
Cells according to claim 1 in which the episomal vector pRPneoU3RHBs.
Claim 4
Dependent Claim
15 words
Episomal vector pRPneoU3RX where CX represents a gene under the control of the HIV-1 LTR.
Claim 5
Dependent Claim
10 words
Vector according to claim 4, in which X is CAT.
Claim 6
Dependent Claim
10 words
Vector according to claim 4, in which X is HBsAG.
Claim 7
Dependent Claim
5 words
Integration vector pSU3R tat .
Claim 8
Dependent Claim
53 words
A process for the preparation of cells according to claims 1-3 in which human 293 cells, transformed by the precocious adenovirus 5 region, are co-transfected with the integration vector pSV3 tat and with pHEBo and then transformed with the episomal vector pRPneoU3RX where X is a gene under the control of HIV-1 LTR.
Claim 9
Dependent Claim
12 words
Process according to claim 8 in which the episomal vector is pRPneoU3RCAT.
Claim 10
Dependent Claim
12 words
Process according to claim 8 in which the episomal vector is RPneoU3RHBs.
Claim 11
Dependent Claim
13 words
The acetyl-transferase (CAT) chloramphenicol enzyme produced with the cells according to claim 1.
Claim 12
Dependent Claim
14 words
Hepatitis B virus surface antigen (HBsAg) produced with the cells according to claim 1.
References Cited (Backward Citations)
No citation records indexed.
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