EP0402917A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Biosensors employing electrical, optical and mechanical signals
Assignee: Biocircuits Corp
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Filing: 1990-06-13
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Granted: —
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Expires: 2010-06-13
Abstract
Abstract Biosensors are provided employing a thin surfactant polymeric electrically conducting layer to which may be bound members of specific binding pairs. Binding of an analyte or a reagent to the specific binding pair member layer may change the electrical, optical, or structural properties of the layer for measurement of analyte. The change in the polymeric layer provides for a sensitive measurement.
Inventors
- 👤 Hans O. Ribi
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 16:23
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; G01N33/5438; B; B01; B01J; B01J2219/00; B01J2219/00274; B01J2219/00718; B01J2219/0072; B01J2219/00734; Y; Y10; Y10S; Y10S436/00; Y10S436/806
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
96 words
A biosensor comprising a plurality of layers: a first electrically non-conductive support providing mechanical rigidity to said biosensor comprising at least two spaced apart electrical leads on a first surface of said support; an electrically conducting organic polymerized surfactant layer in electrical contact with said electrical leads and coating said first surface between said leads; a sealing coating to protect said electrical contacts from contact with water; an organic molecule layer bound to said polymerized surfactant layer binding comprising a member of a specific binding pair for binding to a complementary member in an aqueous medium.
Claim 2
Dependent Claim
96 words
A biosensor comprising a plurality of layers: a first electrically non-conductive support providing mechanical rigidity to said biosensor comprising at least two spaced apart electrical leads on a first surface of said support; an electrically conducting organic polymerized surfactant layer in electrical contact with said electrical leads and coating said first surface between said leads; a sealing coating to protect said electrical contacts from contact with water; an organic molecule layer bound to said polymerized surfactant layer binding comprising a member of a specific binding pair for binding to a complementary member in an aqueous medium.
Claim 3
Dependent Claim
14 words
A biosensor according to Claim 1, wherein said bound organic molecule is a receptor.
Claim 4
Dependent Claim
16 words
A biosensor according to Claim 1, wherein said polymerized lipid layer is comprised of surfactant diynes.
Claim 5
Dependent Claim
21 words
A biosensor according to Claim 3, wherein said surfactant diynes have an aliphatic chain of from 6 to 100 carbon atoms.
Claim 6
Dependent Claim
18 words
A biosensor according to Claim 1, wherein said support is transparent and the opposing second face is reflective.
Claim 7
Dependent Claim
117 words
A biosensor comprising a plurality of layers: a first electrically non-conductive support providing mechanical rigidity to said biosensor comprising at least two spaced apart electrical leads coated on a first surface of said support; an electrically conducting organic polymerized surfactant layer having surfactant moieties of at least 12 carbon atoms in electrical contact with said electrical leads and coating said first surface between said leads, said organic polymerized surfactant layer produced by the polymerization of surfactant diynes; a sealing coating to protect said electrical contacts from contact with water; an organic molecule layer bound to said polymerized surfactant layer comprising a member of a specific binding pair for binding to a complementary member in an aqueous medium.
Claim 8
Dependent Claim
117 words
A biosensor comprising a plurality of layers: a first electrically non-conductive support providing mechanical rigidity to said biosensor comprising at least two spaced apart electrical leads coated on a first surface of said support; an electrically conducting organic polymerized surfactant layer having surfactant moieties of at least 12 carbon atoms in electrical contact with said electrical leads and coating said first surface between said leads, said organic polymerized surfactant layer produced by the polymerization of surfactant diynes; a sealing coating to protect said electrical contacts from contact with water; an organic molecule layer bound to said polymerized surfactant layer comprising a member of a specific binding pair for binding to a complementary member in an aqueous medium.
Claim 9
Dependent Claim
18 words
A biosensor according to Claim 6, wherein said organic molecule layer is a substantially oriented layer of proteins.
Claim 10
Independent Claim
239 words
A method for detecting an analyte in an assay medium, employing as a biosensor a device comprising a plurality of layers: a first electrically non-conductive support providing mechanical rigidity to said biosensor comprising at least two spaced apart electrical leads on a first surface of said support; an electrically conducting organic polymerized surfactant layer in electrical contact with said electrical leads and coating said first surface between said leads; a sealing coating to protect said electrical contacts from contact with water; an organic molecule layer bound to said polymerized surfactant layer-binding comprising a member of a specific binding pair for binding to a complementary member in a fluid medium; said method comprising: adding to said organic molecule layer an assay medium suspected of containing an analyte which is the complementary member of said specific binding pair, with the proviso that binding of said analyte to said organic molecule layer results in a perturbation of said polymerized surfactant layer or a reagent is added which binds to said analyte or said organic molecule layer in proportion to the amount of analyte in said assay medium to cause a perturbation in said polymerized surfactant layer, wherein said perturbation results in a change in the electrical or optical properties of said polymerized surfactant layer; and detecting the change in the electrical or optical properties of said polymerized surfactant layer as an indication of the presence of said analyte in said assay medium.
Claim 11
Independent Claim
239 words
A method for detecting an analyte in an assay medium, employing as a biosensor a device comprising a plurality of layers: a first electrically non-conductive support providing mechanical rigidity to said biosensor comprising at least two spaced apart electrical leads on a first surface of said support; an electrically conducting organic polymerized surfactant layer in electrical contact with said electrical leads and coating said first surface between said leads; a sealing coating to protect said electrical contacts from contact with water; an organic molecule layer bound to said polymerized surfactant layer-binding comprising a member of a specific binding pair for binding to a complementary member in a fluid medium; said method comprising: adding to said organic molecule layer an assay medium suspected of containing an analyte which is the complementary member of said specific binding pair, with the proviso that binding of said analyte to said organic molecule layer results in a perturbation of said polymerized surfactant layer or a reagent is added which binds to said analyte or said organic molecule layer in proportion to the amount of analyte in said assay medium to cause a perturbation in said polymerized surfactant layer, wherein said perturbation results in a change in the electrical or optical properties of said polymerized surfactant layer; and detecting the change in the electrical or optical properties of said polymerized surfactant layer as an indication of the presence of said analyte in said assay medium.
Claim 12
Dependent Claim
21 words
A method according to Claim 8, wherein said analyte is a protein and said specific binding pair member is a hapten.
Claim 13
Dependent Claim
20 words
A method according to Claim 8, wherein said reagent is particles comprising a plurality of molecules cross-reactive with said analyte.
References Cited (Backward Citations)
No citation records indexed.
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