EP0397633A2
Expired / Lapsed
EP
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Fibronectin binding protein as well as its preparation
Assignee: Alfa Laval Agri International AB
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Filing: 1990-05-04
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Granted: —
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Expires: 2010-05-04
Abstract
Abstract The present invention relates to a new recombinant hybrid-DNA-molecule comprising a nucleotide sequence from S. aureus coding for a protein, or polypeptide, having fibronectin binding properties.
Inventors
- 👤 Magnus Höök
- 👤 Klas Jönsson
- 👤 Kjell Martin Lindberg
- 👤 Lars Christer Signäs
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 14:22
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/195; C07K14/305; C07K14/31; A; A61; A61P; A61P17/00; A61P43/00; C07K14/435; C07K14/705; C07K14/70546; A61K; A61K38/00; A61K39/00; Y; Y10; Y10S; Y10S530/00; Y10S530/82; Y10S530/825
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
18 words
Hybrid-DNA-molecule comprising a nucleotide sequence from S. aureus coding for a protein or polypeptide having fibronectin binding activity.
Claim 2
Dependent Claim
18 words
Hybrid-DNA-molecule comprising a nucleotide sequence from S. aureus coding for a protein or polypeptide having fibronectin binding activity.
Claim 3
Dependent Claim
20 words
Plasmid or phage comprising a nucleotide sequence from S. aureus coding for a protein or polypeptide having fibronectin binding activity.
Claim 4
Dependent Claim
20 words
Plasmid or phage comprising a nucleotide sequence from S. aureus coding for a protein or polypeptide having fibronectin binding activity.
Claim 5
Dependent Claim
17 words
A plasmid pFR001 as contained in the E. coli strain 259 having the deposit number DSM 4124.
Claim 6
Dependent Claim
17 words
A plasmid pFR001 as contained in the E. coli strain 259 having the deposit number DSM 4124.
Claim 7
Dependent Claim
9 words
An E. coli strain expressing said fibronectin binding protein.
Claim 8
Dependent Claim
9 words
An E. coli strain expressing said fibronectin binding protein.
Claim 9
Dependent Claim
10 words
A microorganim transformed by recombinant DNA molecule of claims 1-3.
Claim 10
Dependent Claim
14 words
Hybrid-DNA-molecule according to claim 1, characterized in that it comprises the following nucleotide sequence:
Claim 11
Dependent Claim
13 words
Plasmid or phage comprising one or more nucleotide sequences according to claim 6.
Claim 12
Dependent Claim
12 words
Microorganism containing at least a plasmid or phage according to claim 7.
Claim 13
Dependent Claim
64 words
A method for producing a fibronectin binding protein or polypeptide, wherein a) at least one hybrid-DNA-molecule according to claim 1 is introduced into a microorganism, b) said microorganism is cultivated in a growth promoting medium, and c) the protein thus formed is isolated by means of an affinity chromatography on a coloumn with fibronectin bound to an insolubilized carrier followed by ion exchange chromatography.
Claim 14
Dependent Claim
67 words
Chemical synthesis method for producing a fibronectin binding protein or polypeptide according to claim 1, whereby an amino acid residue is built up based on said nucleotide sequence encoding for said protein or polypeptide starting from the C-terminal alanine, which is stepwise reacted with the appropriate amino acid, whereby it is finally reacted with isoleucine at the N-terminal end, to form the fibronectin binding protein or polypeptide.
Claim 15
Dependent Claim
15 words
A fibronectin binding protein or polypeptide comprising at least one of the amino acid sequence
Claim 16
Dependent Claim
15 words
A fibronectin binding protein or polypeptide comprising at least one of the amino acid sequence
References Cited (Backward Citations)
No citation records indexed.
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