EP0362526A2
Expired / Lapsed
EP
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The Alpha-subunit of the LFA-1 leukocyte adhesion receptor
Assignee: Dana Farber Cancer Institute Inc
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Filing: 1989-08-17
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Granted: —
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Expires: 2009-08-17
Abstract
Abstract The present invention relates to LFA-1 alpha-subunit which is involved in the process through which cell recognize and migrate to sites of inflammation as well as attach to cellular substrates during inflammation. The invention is directed toward such molecules, the functional derivatives of such molecules, screening assays for identifying such molecules and therapeutic and diagnostic uses for such molecules.
Inventors
- 👤 Timothy Alan Springer
- 👤 Richard Larson
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 09:37
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/705; C07K14/70546; C07K14/70553; A; A61; A61P; A61P17/00; A61P29/00; A61P35/00; A61P37/00; A61P37/08; A61K; A61K38/00
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
12 words
LFA-1 alpha-subunit, or a functional derivative thereof, substantially free of natural contaminants.
Claim 2
Dependent Claim
12 words
LFA-1 alpha-subunit, or a functional derivative thereof, substantially free of natural contaminants.
Claim 3
Dependent Claim
30 words
The LFA-1 alpha-subunit or said functional derivative thereof of claim 1, wherein said LFA-1 alpha-subunit is additionally capable of binding to a molecule present on the surface of a cell.
Claim 4
Dependent Claim
24 words
The LFA-1 alpha-subunit of claim 2, wherein said molecule present on the surface of said cell is ICAM-1 or another natural ligand of LFA-1.
Claim 5
Dependent Claim
23 words
The LFA-1 alpha-subunit of claim 1, wherein said molecule is capable of associating with an LFA-1 beta subunit to form an LFA-1 heterodimer.
Claim 6
Dependent Claim
22 words
The LFA-1 alpha-subunit of claim 4, wherein said LFA-1 heterodimer is capable of binding to ICAM-1 or another natural ligand of LFA-1.
Claim 7
Dependent Claim
51 words
The LFA-1 alpha-subunit molecule of claim 2, wherein said molecule additionally contains at least one polypeptide selected from the group consisting of: a. P-P-R-A-G-R-H; b. I-I-T-D-G-E-A; c. D-W-A-G-G-F-L; d. S-Q-V-Q-T-I-H; e. R-H-G-G-L-S-P; f. M-S-C-T-D-F-S; g. R-L-L-S-R-A-L; h. G-V-D-V-Q-D-G-E-I-E; i. D-I-N-G-D-G-L-V-D-V; j. V-K-D-L-E-G-D-G-L-A; k. T-Y-L-S-G-L; 1. Y-I-I-G-I-G-K; m. I-E-G-T-Q-V-L-S-Q; and n. P-S-I-H-N-I-P.
Claim 8
Dependent Claim
21 words
The functional derivative of the LFA-1 alpha-subunit of claim 2 wherein said functional derivative is a fragment of the LFA-1 alpha-subunit.
Claim 9
Dependent Claim
20 words
The functional derivative of the LFA-1 alpha-subunit of claim 2 wherein said functional derivative is a variant of LFA-1 alpha-subunit.
Claim 10
Dependent Claim
20 words
The functional derivative of the LFA-1 alpha-subunit of claim 2 wherein said functional derivative is an analog of LFA-1 alpha-subunit.
Claim 11
Dependent Claim
21 words
The functional derivative of the LFA-1 alpha-subunit of claim 2 wherein said functional derivative is a chemical derivative of LFA-1 alpha-subunit.
Claim 12
Dependent Claim
16 words
A recombinant DNA molecule capable of expressing either the LFA-1 alpha-subunit or a functional derivative thereof.
Claim 13
Dependent Claim
16 words
A recombinant DNA molecule capable of expressing either the LFA-1 alpha-subunit or a functional derivative thereof.
Claim 14
Dependent Claim
55 words
The DNA molecule of claim 11, wherein said LFA-1 alpha-subunit or said functional derivative thereof contains at least one poly-peptide selected from the group consisting of: a. P-P-R-A-G-R-H; b. I-I-T-D-G-E-A; c. D-W-A-G-G-F-L; d. S-Q-V-Q-T-I-H; e. R-H-G-G-L-S-P; f. M-S-C-T-D-F-S; g. R-L-L-S-R-A-L; h. G-V-D-V-Q-D-G-E-I-E; i. D-I-N-G-D-G-L-V-D-V; j. V-K-D-L-E-G-D-G-L-A; k. T-Y-L-S-G-L; 1. Y-I-I-G-I-G-K; m. I-E-G-T-Q-V-L-S-Q; and n. P-S-I-H-N-I-P.
Claim 15
Dependent Claim
53 words
An LFA-1 alpha-subunit or functional derivative thereof for the use in the suppression of inflammation, the suppression of metastasis of an hematopoietic tumor cell which requires a functional member of the LFA-1 family for migration, the suppression of growth of an LFA-1 alpha-subunit expressing tumor cell or the treatment of a viral infection.
Claim 16
Dependent Claim
53 words
An LFA-1 alpha-subunit or functional derivative thereof for the use in the suppression of inflammation, the suppression of metastasis of an hematopoietic tumor cell which requires a functional member of the LFA-1 family for migration, the suppression of growth of an LFA-1 alpha-subunit expressing tumor cell or the treatment of a viral infection.
Claim 17
Independent Claim
93 words
A method of diagnosing the presence and location of inflammation resulting from a response of the specific defense system in a mammalian subject suspected of having said inflammation which comprises: (a) incubating a sample of tissue of said subject with a composition containing a nucleic acid molecule capable of binding to a molecule selected from the group consisting of a DNA sequence of LFA-1 alpha-subunit, wherein said binding is capable of identifying a cell which is capable of binding ICAM-1, or another natural ligand of LFA-1, and (b) detecting said nucleic acid molecule.
Claim 18
Independent Claim
93 words
A method of diagnosing the presence and location of inflammation resulting from a response of the specific defense system in a mammalian subject suspected of having said inflammation which comprises: (a) incubating a sample of tissue of said subject with a composition containing a nucleic acid molecule capable of binding to a molecule selected from the group consisting of a DNA sequence of LFA-1 alpha-subunit, wherein said binding is capable of identifying a cell which is capable of binding ICAM-1, or another natural ligand of LFA-1, and (b) detecting said nucleic acid molecule.
Claim 19
Independent Claim
68 words
A method of diagnosing the presence and location of inflammation resulting from a response of the specific defense system in a mammalian subject suspected of having said inflammation which comprises: (a) incubating a sample of tissue of said subject with a composition containing a detectably labeled LFA-1 alpha-subunit capable of identifying a cell which expresses ICAM-1, or another natural ligand of LFA-1, and (b) detecting said LFA-1 alpha-subunit.
Claim 20
Independent Claim
68 words
A method of diagnosing the presence and location of inflammation resulting from a response of the specific defense system in a mammalian subject suspected of having said inflammation which comprises: (a) incubating a sample of tissue of said subject with a composition containing a detectably labeled LFA-1 alpha-subunit capable of identifying a cell which expresses ICAM-1, or another natural ligand of LFA-1, and (b) detecting said LFA-1 alpha-subunit.
Claim 21
Independent Claim
101 words
A method of diagnosing the presence and location of a tumor cell which expresses ICAM-1 or another natural ligand of LFA-1, in a subject suspected of having such a cell, which comprises: (a) incubating a sample of tissue of said subject in the presence of a composition containing a detectably labeled binding ligand capable of binding to said ICAM-1, or another natural ligand of LFA-1, said ligands being selected from the group consisting of LFA-1 alpha-subunit and a functional derivative of LFA-1 alpha-subunit, and (b) detecting said binding ligand which is bound to said ICAM-1 present in said sample of tissue.
Claim 22
Independent Claim
101 words
A method of diagnosing the presence and location of a tumor cell which expresses ICAM-1 or another natural ligand of LFA-1, in a subject suspected of having such a cell, which comprises: (a) incubating a sample of tissue of said subject in the presence of a composition containing a detectably labeled binding ligand capable of binding to said ICAM-1, or another natural ligand of LFA-1, said ligands being selected from the group consisting of LFA-1 alpha-subunit and a functional derivative of LFA-1 alpha-subunit, and (b) detecting said binding ligand which is bound to said ICAM-1 present in said sample of tissue.
Claim 23
Independent Claim
33 words
A method of treating viral infections which comprises providing to a subject in need of such treatment, an effective amount of a composition comprising the alpha-subunit of LFA-1, or a functional derivative thereof.
Claim 24
Independent Claim
33 words
A method of treating viral infections which comprises providing to a subject in need of such treatment, an effective amount of a composition comprising the alpha-subunit of LFA-1, or a functional derivative thereof.
References Cited (Backward Citations)
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