EP0330389A2
Expired / Lapsed
EP
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Human collagen processing and autoimplant use
Assignee: Autogenesis Technologies Inc
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Filing: 1989-02-17
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Granted: —
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Expires: 2009-02-17
Abstract
Abstract Production of a chemically modified, crosslinkable, telopeptide-containing, naturally crosslinked, solubilized collagen from tissue obtained from a sole human donor, for implanting in the same donor, by chemically modifying the tissue, e.g. by acylation and/or esterification, to form an autoimplantable, crosslinkable, telopeptide-containing, naturally crosslinked, collagen product, as a completely solubilized collagen solution, optionally of high index of refraction for correcting sight, or as a partially solubilized collagen suspension; and use of the product for altering the condition of in situ tissue of the same human donor by autoimplantation, such as by crosslinking the product to form an implant device, by placing an effective amount of the product at the tissue site and crosslinking the product in situ, or by partially crosslinking the product and placing an effective amount thereof at the tissue site and optionally further crosslinking the product in situ.
Inventors
- 👤 CHARLES D. kELMAN
- 👤 Dale P. Devore
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 00:33
CPC Classifications
C; C08; C08H; C08H1/00; C08H1/06; A; A61; A61L; A61L27/00; A61L27/14; A61L27/22; A61L27/24; C07; C07K; C07K14/00; C07K14/435; C07K14/78; A61F; A61F2310/00; A61F2310/00005; A61F2310/00365; A61K; A61K38/00; Y; Y10; Y10S; Y10S128/00; Y10S128/08; Y10S623/00; Y10S623/915
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
24 words
A composition characterised in that it comprises a backbone of telopeptide-containing collagen, derived from human tissue, having acylated amine groups and/or esterified carboxyl groups.
Claim 2
Dependent Claim
24 words
A composition characterised in that it comprises a backbone of telopeptide-containing collagen, derived from human tissue, having acylated amine groups and/or esterified carboxyl groups.
Claim 3
Dependent Claim
36 words
A composition according to Claim 1 characterised in that the acylated amine groups have aliphatic carbonyl, aromatic carbonyl, aliphatic sulfonyl or aromatic sulfonyl acyl moieties, or the esterified carboxyl groups have aliphatic or aromatic ester moieties.
Claim 4
Dependent Claim
50 words
A composition according to Claim 1 or Claim 2 characterised in that the said collagen is combined with a substantially neutral aqueous liquid to form a substantially homogenous solution in which any said collagen which remains undissolved is present as suspended material having acylated amine groups and/or esterified carboxyl groups.
Claim 5
Dependent Claim
31 words
A composition according to any of the preceding claims, characterised in that the said backbone is crosslinked through said acylated amine groups and/or said esterified carboxyl groups to another said backbone.
Claim 6
Dependent Claim
48 words
A composition according to any of the preceding claims, characterised in that the backbone of telopeptide-containing collagen comprises triple helix strands of telopeptide-containing collagen, derived from human tissue obtained from a sole donor, including polypeptide backbones having appended thereto the said acylated amine groups and/or esterified carboxyl groups.
Claim 7
Dependent Claim
36 words
A composition according to Claim 5 characterised in that the said backbones have appended thereto acylated amine groups in which the acyl moieties thereof possess an index of refraction of at least about n D 1.500.
Claim 8
Dependent Claim
16 words
A shaped article characterised in that it contains the composition of any of the preceding claims.
Claim 9
Dependent Claim
101 words
A process for preparing a crosslinkable, telopeptide-containing collagen from intact human tissue, characterised in that it comprises reacting said tissue (a) with an amine reactive acylating agent at substantially neutral to basic pH in aqueous medium sufficiently to form an at least partially solubilized telopeptide-containing collagen having crosslinkable acylated amine groups and which is at least partially dissolved in the medium, and/or (b) with a carboxylic acid reactive esterifying agent at acidic pH in organic medium sufficiently to form an at least partially solubilized telopeptide-containing collagen having crosslinkable esterified carboxyl groups and which is at least partially dissolved in the medium.
Claim 10
Dependent Claim
101 words
A process for preparing a crosslinkable, telopeptide-containing collagen from intact human tissue, characterised in that it comprises reacting said tissue (a) with an amine reactive acylating agent at substantially neutral to basic pH in aqueous medium sufficiently to form an at least partially solubilized telopeptide-containing collagen having crosslinkable acylated amine groups and which is at least partially dissolved in the medium, and/or (b) with a carboxylic acid reactive esterifying agent at acidic pH in organic medium sufficiently to form an at least partially solubilized telopeptide-containing collagen having crosslinkable esterified carboxyl groups and which is at least partially dissolved in the medium.
Claim 11
Dependent Claim
105 words
A process according to Claim 8 characterised in that (a) the reaction with the acylating agent is carried out at about 0 to 45°C and at a weight ratio of the acylating agent to the wet tissue of about 0.005 to 0.5 : 1 while maintaining the aqeous medium at about pH 7 to 11, or (b) the reaction with the esterifying agent is carried out at about 0 to 45°C and at a weight ratio of the esterifying agent to the dry tissue of about 1 to 30 : 1 in nonaqueous organic medium in the presence of a catalytic amount of an acid.
Claim 12
Dependent Claim
46 words
A process according to Claim 8 or Claim 9, characterised in that the acylating agent is an aliphatic- or aromatic- carboxylic acid anhydride, carboxylic acid ester, carboxylic acid halide, sulfonic acid or sulfonic acid halide, or the esterifying agent is an acidified aliphatic or aromatic alcohol.
Claim 13
Dependent Claim
27 words
A process according to any of Claims 8 to 10, characterised in that attendant noncollagenous protein contaminates are preliminarily removed from the tissue prior to the reaction.
Claim 14
Dependent Claim
69 words
A process according to Claim 11, characterised in that the contaminates are removed by contacting the tissue at a temperature not greater than about room temperature with a solubilizing liquid of substantially neutral pH which is capable of solubilizing the contaminates without solubilizing the collagen, while pulverizing the tissue, to form essentially noncollagenous protein contaminate-free pulverized tissue, and the contaminate-free tissue is thereafter reacted to form said telopeptide-containing collagen.
Claim 15
Dependent Claim
26 words
A process according to any of Claims 8 to 12, characterised in that attendant lipid constituents are preliminarily removed from the tissue prior to the reaction.
Claim 16
Dependent Claim
57 words
A process according to any of Claims 8 to 13, characterised in that the said tissue has been obtained from a sole donor, the reaction is carried out until the collagen is substantially completely solubilized in the medium, and the solubilized collagen is recovered, purified and combined with an aqueous liquid to form a telopeptide-containing collagen solution.
Claim 17
Dependent Claim
115 words
A process according to any of Claims 8 to 14, characterised in that the said tissue has been obtained from a sole donor, the agent is an amine reactive acylating agent which has a selective index of refraction of from about n D 1.500 to about n D 1.600 and is capable of modifying the collagen to provide completely solubilized collagen with a high index of refraction effective for correcting sight, the reaction is carried out until the collagen is substantially completely solubilized in the aqueous medium, and the solubilized collagen is recovered, purified and combined with an aqueous liquid to form a telopeptide-containing collagen solution, of selective high index of refraction for correcting sight.
Claim 18
Dependent Claim
25 words
A process according to Claim 15, characterised in that the said agent is aniline-2-sulfonic acid, 3-nitrobenzene-sulfonic acid, 2-formylbenzene-sulfonic acid, 1,3-benzene-disulfonic acid, or 1,2,4,5-benzene-tetracarboxylic acid dianhydride.
Claim 19
Dependent Claim
95 words
A process according to any of Claims 8 to 13, characterised in that the said tissue has been obtained from a sole donor, the reaction is carried out until the collagen is only partially solubilized in the medium to provide a mixture containing suspendable fine fibrous particles of unsolubilized telopeptide-containing collagen in a homogenous mass of solubilized telopeptide-containing collagen, and the suspendable fine particles and solubilized collagen are recovered, purified and combined with an aqueous liquid to form a substantially homogeneous solution including suspended telopeptide-containing collagen material having acylated amine groups or esterified carboxyl groups.
Claim 20
Dependent Claim
49 words
A process for producing a telopeptide-containing collagen implant device characterised in that it comprises forming a composition as claimed in any of Claims 1 to 6 into a mass of selective shape and size corresponding to an effective implant device, and thereafter crosslinking said mass to produce said device.
References Cited (Backward Citations)
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