Patent DE594182C successfully refreshed from Google Patents.
EP0325262A2
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EP
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Cloned genes encoding IG-CD4 fusion proteins and the use thereof
Assignee: General Hospital Corp
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Filing: 1989-01-20
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Granted: —
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Expires: 2009-01-20
Abstract
Abstract Fusion proteins of immunoglobulins of the IgM, IgG1 or IgG3 class, wherein the variable region of the light or heavy chain has been replaced with CD4 or fragments thereof capable of binding to gp120 or immunoglobulin-like molecules comprising such fusion proteins together with an immunoglobulin light or heavy chain can be administered to an animal suffering from HIV or SIV infection. They also are useful in assays for HIV or SIV comprising contacting a sample suspected of containing HIV or SIV gp120 with the immunoglobulin-like molecule or fusion protein, and detecting whether a complex is formed.
Inventors
- 👤 Seed Dr. Brian
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 16:34
CPC Classifications
C; C12; C12N; C12N15/00; C07; C07K; C07K14/00; C07K14/435; C07K14/705; C07K14/70503; C07K14/70514; A; A61; A61P; A61P31/00; A61P31/12; C07K16/00; C07K2319/00; C07K2319/01; C07K2319/036; C07K2319/32; C07K2319/33
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
60 words
A fusion protein gene comprising 1) the DNA sequence of CD4, or fragment thereof which binds to HIV gp120, and 2) the DNA sequence of an immunoglobulin heavy chain, characterized in that the DNA sequence which encodes the variable region of said immunoglobulin chain has been replaced with the DNA sequence which encodes CD4, or said gp120 binding fragment thereof.
Claim 2
Dependent Claim
60 words
A fusion protein gene comprising 1) the DNA sequence of CD4, or fragment thereof which binds to HIV gp120, and 2) the DNA sequence of an immunoglobulin heavy chain, characterized in that the DNA sequence which encodes the variable region of said immunoglobulin chain has been replaced with the DNA sequence which encodes CD4, or said gp120 binding fragment thereof.
Claim 3
Dependent Claim
37 words
The fusion protein gene of claim 1, wherein the DNA sequence which encodes said fragment of CD4 comprises the following DNA sequence: or a degenerate variant thereof, or the following DNA sequence: or a degenerate variant thereof.
Claim 4
Dependent Claim
23 words
The fusion protein gene of claim 1 or 2, characterized in that said immunoglobulin chain is of the class IgM, IgG1 or IgG3.
Claim 5
Dependent Claim
60 words
A fusion protein gene comprising 1) the DNA sequence of CD4, or fragment thereof which binds to HIV gp120, and 2) the DNA sequence of an immunoglobulin light chain, characterized in that the DNA sequence which encodes the variable region of said immunoglobulin light chain has been replaced with the DNA sequence which encodes CD4, or HIV gp120-binding fragment thereof.
Claim 6
Dependent Claim
60 words
A fusion protein gene comprising 1) the DNA sequence of CD4, or fragment thereof which binds to HIV gp120, and 2) the DNA sequence of an immunoglobulin light chain, characterized in that the DNA sequence which encodes the variable region of said immunoglobulin light chain has been replaced with the DNA sequence which encodes CD4, or HIV gp120-binding fragment thereof.
Claim 7
Dependent Claim
39 words
A fusion protein gene of claim 4, characterized in that the DNA sequence which encodes said fragment of CD4 comprises the following DNA sequence: or a degenerate variant thereof, or the following DNA sequence: or a degenerate variant thereof.
Claim 8
Dependent Claim
73 words
A vector comprising the fusion protein gene of claim 1, preferably having the identifying characteristics of pCD4Hy1, which has been deposited under Accession No. 67611, or pCD4Mu., which has been deposited under Accession No. 67608, or of pCD4Pu., which has been deposited under Accession No. 67609, or of pCD4E γ 1, which has been deposited under Accession No. 67610, all in E. coli at the ATCC under the terms of the Budapest Treaty.
Claim 9
Dependent Claim
10 words
A vector comprising the fusion protein gene of claim 4.
Claim 10
Dependent Claim
11 words
A host transformed with the vector of claim 6 or 7.
Claim 11
Dependent Claim
57 words
The host of claim 8 which expresses an immunoglobulin light chain together with the expression product of said fusion protein gene to give an immunoglobulin-like molecule which binds to gp120 or an immunoglobulin heavy chain together with the expression product of said fusion protein gene to give an immunoglobulin-like molecule which binds to HIV or SIV gp120.
Claim 12
Dependent Claim
19 words
The host of claim 9, wherein said immunoglobulin heavy chain is of the immunoglobulin class IgM, IgG1 or IgG3.
Claim 13
Dependent Claim
90 words
A method of producing a fusion protein comprising CD4, or fragment thereof which binds to gp120, and immunoglobulin heavy chain, wherein the variable region of the immunoglobulin chain has been substituted with CD4, or fragment thereof which binds to HIV or SIV gp120, characterized by cultivating in a nutrient medium under protein-producing conditions, a host strain transformed with the vector of claim 6, said vector further comprising expression signals which are recognized by said host strain and direct expression of said fusion protein, and recovering the fusion protein so produced.
Claim 14
Dependent Claim
45 words
The method of claim 11, wherein said host strain is a myeloma cell line which produces immunoglobulin light chains and said fusion protein comprises an immunoglobulin heavy chain of the class IgM, IgG1 or IgG3, wherein an immunoglobulin-like molecule comprising said fusion protein is produced.
Claim 15
Dependent Claim
91 words
A method of producing a fusion protein comprising CD4, or fragment thereof which binds to gp120, and an immunoglobulin light chain, wherein the variable region of the immunoglobulin chain has been substituted with CD4, or fragment thereof which binds to HIV or SIV gp120, characterized by cultivating in a nutrient medium under protein-producing conditions, a host strain transformed with the vector of claim 7, said vector further comprising expression signals which are recognized by said host strain and direct expression of said fusion protein, and recovering the fusion protein so produced.
Claim 16
Dependent Claim
33 words
The method of claim 13, wherein said host produces immuno-globulin heavy chains of the class IgM, IgG1 and IgG3 together with said fusion protein to give an immunoglobulin-like molecule which binds to HIV-gp120.
Claim 17
Dependent Claim
80 words
A fusion protein, which is preferably detectably labeled, comprising CD4, or fragment thereof which is capable of binding to HIV or SIV gp120, fused at the C-terminus to a second protein which comprises an immunoglobulin heavy chain of the class IgM, igG1 or IgG3, wherein the variable region of said heavy chain immunoglobulin has been replaced with CD4, or HIV gp120-binding fragment thereof, and preferably further comprising a therapeutic agent, radiolabel or NMR imaging agent linked to said fusion protein.
Claim 18
Dependent Claim
80 words
A fusion protein, which is preferably detectably labeled, comprising CD4, or fragment thereof which is capable of binding to HIV or SIV gp120, fused at the C-terminus to a second protein which comprises an immunoglobulin heavy chain of the class IgM, igG1 or IgG3, wherein the variable region of said heavy chain immunoglobulin has been replaced with CD4, or HIV gp120-binding fragment thereof, and preferably further comprising a therapeutic agent, radiolabel or NMR imaging agent linked to said fusion protein.
Claim 19
Dependent Claim
13 words
The fusion proteins CD4Hy1, CD4Ma, CD4Pµ, CD4E γ 1 or CD4B γ 1.
Claim 20
Dependent Claim
13 words
The fusion proteins CD4Hy1, CD4Ma, CD4Pµ, CD4E γ 1 or CD4B γ 1.
Claim 21
Dependent Claim
38 words
An immunoglobulin-like molecule, comprising the fusion protein of claim 15 and an immunoglobulin light chain, preferably further comprising a detectable label, and especially further comprising a therapeutic agent, radiolabel or NMR imaging agent linked to said immunoglobulin-like molecule.
Claim 22
Dependent Claim
57 words
A fusion protein comprising CD4, or fragment thereof which binds to HIV gp120, fused at the C-terminus to a second protein comprising an immunoglobulin light chain where the variable region has been deleted, and which fusion protein preferably is detectably labeled, especially further comprising a therapeutic agent, radiolabel or NMR imaging agent linked to said fusion protein.
Claim 23
Dependent Claim
57 words
A fusion protein comprising CD4, or fragment thereof which binds to HIV gp120, fused at the C-terminus to a second protein comprising an immunoglobulin light chain where the variable region has been deleted, and which fusion protein preferably is detectably labeled, especially further comprising a therapeutic agent, radiolabel or NMR imaging agent linked to said fusion protein.
Claim 24
Dependent Claim
22 words
The fusion protein of claim 15, wherein said CD4 fragment comprises the following amino acid sequence: or the following amino acid sequence:
Claim 25
Dependent Claim
45 words
An immunoglobulin-like molecule comprising the fusion protein of claim 18 and an immunoglobulin heavy chain of the class IgM, IgG1 or IgG3, preferably further comprising a detectable label, and especially further comprising a therapeutic agent, radiolabel or NMR imaging agent linked to said immunoglobulin-like molecule.
Claim 26
Dependent Claim
16 words
A complex comprising the fusion protein of claim 15 or 18 and HIV or SIV gp120.
Claim 27
Dependent Claim
32 words
The complex of claim 21, wherein said gp120 is a part of an HIV or SIV, is expressed on the surface of an HIV or SIV-infected cell or is present in solution.
Claim 28
Dependent Claim
50 words
A method for the detection of HIV or SIV gp120 in a sample, characterized by (a) contacting a sample suspected of containing HIV or SIV gp120 with the fusion protein of claim 15 or 18, and (b) detecting whether a complex is formed, said fusion protein preferably being detectably labeled.
References Cited (Backward Citations)
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