Immunosensor

Claim 2

An sensor means for conducting immunoassays comprising: a film of semiconductive polymer having an obverse and reverse surface, a common electroconductive area on the reverse sur­face incontact therewith, at least one further electroconductive area of the same electroconductive material as in said common area on said reverse surface and in contact therewith, at least one second further electroconductive area placed at a different location on the reverse surface and in contact therewith, means for separating the obverse of the film in such a manner that the a first field is defined over said first electroconductive area and a second field is defined over said second electroconductive area while a portion of each of said fields lies over the said common electroconductive area.

Claim 3

An sensor means of Claim 1 comprising: a film of semiconductive polymer having an obverse and reverse surface, a narrow electroconductive strip on the reverse sur­face incontact therewith, said strip dividing said film into two fields of equal area, at least one further electroconductive area of the same electroconductive material as the said strip placed on said reverse surface and in contact therewith, on one field, at least one second further electroconductive area placed on the remaining field on the reverse surface and in contact therewith in such a manner that the electrocon­ductive areas on both fields are equally large and equi­distant from the said strip.

Claim 4

An immunoassay sensor cell comprising a sensor means of Claim 1, electrical connection means to said electroconductive areas, a sample reservoir having an open upper and lower end the portion of said reservoir surrounding said open lower end being adapted to contact and form a liquid leakproof seal with the obverse surface of the film, of sufficient size to encompass at least the obverse surface over the electroconductive areas on the reverse side, a dual chamber insert, adapted to fit removably in­side said reservoir, having open upper and lower ends, the portion of said insert surrounding said open lower end being adapted to contact and form a liquid leakproof seal with the obverse surface of the film and being of suffici­ent size to encompass at least a part of the obverse sur­face over the electroconductive areas on the reverse side within said open lower end and having a center partition similarly adapted to contact and form a liquid leakproof seal with the obverse surface of the film.

Claim 5

A device of claim 3 wherein the common electroconductive area is a narrow electroconductive strip on the reverse surface in contact therewith, said strip dividing said film into two fields of equal area, and the first and second other electroconductive areas are at least one further electroconductive area of the same electroconductive material as the said strip placed on said reverse surface and in contact therewith, on one field,and at least one second further electroconductive area placed on the remaining field on the reverse surface and in contact therewith in such a manner that the electrocon­ductive areas on both fields are equally large and equi­distant from the said strip. whereby said central partition defines two fields of equal area on either side of the longitudinal axis of the said strip, upon the obverse side of the film.

Claim 8

A method of Claim 5 wherein the substrate is a semiconductive polymer and the modifiable quality is the conductivity of said substrate.

Claim 9

A method of Claim 6 wherein the polymer is selected from the group consisting of: polyacetylene, polypyrrole, polythiophene, polyparaphenylene and polysulfone.

Claim 10

A method of Claim 7 which further comprises binding a portion of said first component of said substrate modifying agent directly to the first predetermined portion of the substrate, contacting both the first predetermined portion of the substrate having the binding agent bound thereto, and the second predetermined portion having no binding agent bound thereto with said suspected fluid, said substrate modifying agent and a scavenger for said modifying factor, whereby when the first component of the modifying agent is bound to the substrate via the analyte bound to the binding agent on the substrate, the modifying factor generated thereby preferentially modifies the substrate while the remaining modifying factor is reacted with the scavenger.

Claim 11

A process of Claim 8 wherein the substrate is polyacetylene, the substrate modifying agent first component is lactoperoxidase bound to glucose oxidase and the second component thereof is glucose, and the scavenger is a water soluble protein containing substitutable ring aryl groups.