EP0279582A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
DNA sequences to target proteins to the mammary gland for efficient secretion
Assignee: Pharming Intellectual Property BV
•
Filing: 1988-02-10
•
Granted: —
•
Expires: 2008-02-10
Abstract
Abstract Described is a method of targeting specific genes to the mammary gland which results in the efficient synthesis and secretion of biologically important molecules. Further, there is described as a composition of matter, a transgenic mammal having the ability to reproduce itself and being suitable for the secretion of biologically active agents into its milk. Additionally there is disclosed as a composition of matter, recombinant DNA gene complexes designed to integrate into a mammalian genome and to synthesize and secrete biological active agents into the milk. Furthermore methods of producing and using altered milk are disclosed.
Inventors
- 👤 Jeffrey M. Rosen
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 04:57
CPC Classifications
A; A01; A01K; A01K67/00; A01K67/027; A01K67/0275; A01K67/02; A23; A23C; A23C9/00; A23C9/20; A61; A61P; A61P15/00; A61P43/00; C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4732; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/85; C12N15/8509; C12N15/87; C12N15/89; C12N9/00; C12N9/10; C12N9/1025; C12N9/1029; C12N9/1033; A01K2217/00; A01K2217/05; A01K2217/20; A01K2227/00; A01K2227/10; A01K2227/105; A01K2227/108; A01K2267/00; A01K2267/01; A23C2230/00; A23C2230/05; C07K2319/00; C07K2319/01; C07K2319/02; C07K2319/61; C12N2830/00; C12N2830/008; C12N2830/42; C12N2830/80; C12N2830/85
Claims Analysis
Total Claims: 44
Claim 1
Independent Claim
39 words
As a composition of matter, a recombinant DNA gene complex, comprising: (a) a promoter sequence; (b) an enhancer sequence; (c) a signal peptide sequence: and (d) a coding sequence derived from a gene coding for a biological active agent
Claim 2
Dependent Claim
39 words
As a composition of matter, a recombinant DNA gene complex, comprising: (a) a promoter sequence; (b) an enhancer sequence; (c) a signal peptide sequence: and (d) a coding sequence derived from a gene coding for a biological active agent
Claim 3
Dependent Claim
32 words
wherein said promoter sequence, said enhancer sequence and said signal peptide sequence derive from at least one mammary gland-specific gene and facilitate the expression of said coding sequence in the mammary gland.
Claim 4
Dependent Claim
32 words
wherein said promoter sequence, said enhancer sequence and said signal peptide sequence derive from at least one mammary gland-specific gene and facilitate the expression of said coding sequence in the mammary gland.
Claim 5
Dependent Claim
36 words
A composition of matter according to Claim 1, wherein said at breast one mammary gland-specific gene is selected from the group consisting of genes coding for α-casein, β-casein, γ-casein, κ-casein, α-lactalbumin, β-lactoglobulin and whey acidic protein.
Claim 6
Dependent Claim
25 words
A composition of matter according to Claim 1, wherein said promoter sequence, said enhancer sequence and said signal peptide sequence derive from the same gene.
Claim 7
Dependent Claim
24 words
A composition of matter according to Claim 3, wherein said promoter sequence, said enhancer sequence and said signal peptide sequence are a β-casein gene.
Claim 8
Dependent Claim
33 words
A composition of matter according to any preceding Claim, wherein said coding sequence is derived from the group consisting of genes coding for hormones, drugs, proteins, lipids, carbohydrates, growth factors, and bacterostatic agents.
Claim 9
Dependent Claim
38 words
A composition of matter according to any of Claims 1 - 4, wherein said coding sequence is derived from the group consisting of genes coding for α-casein, β-casein, γ-casein, κ-casein, α-lactalbumin, β-lactoglobulin, whey acid protein and chloramphenicol acetylictransferase.
Claim 10
Dependent Claim
15 words
A composition of matter according to any preceding Claim, comprising further a glucocorticoid response element.
Claim 11
Dependent Claim
24 words
A composition of matter according to any preceding Claim, further comprising: (a) a 5ʹ untranslated mRNA sequence; and (b) a 3ʹ untranslated mRNA sequence;
Claim 12
Dependent Claim
23 words
wherein said 5ʹ untranslated and said 3ʹ untranslated mRNA sequences are attached to the 5ʹ and 3ʹ ends respectively of the coding sequence.
Claim 13
Dependent Claim
23 words
wherein said 5ʹ untranslated and said 3ʹ untranslated mRNA sequences are attached to the 5ʹ and 3ʹ ends respectively of the coding sequence.
Claim 14
Dependent Claim
35 words
A composition of matter according to Claim 8, wherein said 5ʹ untranslated mRNA sequence and said 3ʹ untranslated mRNA sequence are derived from the group of untranslated mRNA sequences consisting of β-globin, β-casein and vitellogenin.
Claim 15
Dependent Claim
25 words
A composition of matter according to Claim 9, wherein said 5ʹ untranslated mRNA sequence, and said 3ʹ untranslated mRNA sequence derive from the β-casein gene.
Claim 16
Dependent Claim
17 words
A composition of matter according to Claim 8, 9 or 10, comprising further a glucocorticoid response element.
Claim 17
Independent Claim
60 words
A method of constructing a mammary gland specific recombinant DNA gene complex, comprising the step of: linking a promoter sequence, an enhancer sequence, a signal peptide sequence and a coding sequence, wherein said promoter sequence, enhancer sequence and signal peptide sequence are selected from mammary gland-specific genes, and said coding sequence is a gene coding for a biological active agent.
Claim 18
Independent Claim
60 words
A method of constructing a mammary gland specific recombinant DNA gene complex, comprising the step of: linking a promoter sequence, an enhancer sequence, a signal peptide sequence and a coding sequence, wherein said promoter sequence, enhancer sequence and signal peptide sequence are selected from mammary gland-specific genes, and said coding sequence is a gene coding for a biological active agent.
Claim 19
Dependent Claim
34 words
The method of Claim 12, wherein said promoter sequence said enhancer sequence and said signal peptide sequence are selected from the group consisting of α-casein, β-casein, γ-casein, κ-casein, α-lactalbumin, β-lactoglobulin and whey acidic protein.
Claim 20
Dependent Claim
39 words
The method of Claim 13, wherein said promoter sequence, enhancer sequence and signal peptide sequence derive from the β-casein sequence; and said signal peptide sequence facilitates the secretion of the synthesized peptide into the milk of the mammary gland.
Claim 21
Dependent Claim
27 words
The method of Claim 14, wherein said coding sequence is derived from the group consisting of gene coding for hormones, drugs, proteins, lipids, carbohydrates and bacterostatic agents.
Claim 22
Dependent Claim
30 words
The method of Claim 15, wherein said coding sequence is derived from the group consisting of genes coding for chloramphenicol acetyltransferase, β-casein, α-casein, γ-casein, α-lactalbumin, β-lactoglobulin and whey acidic protein.
Claim 23
Independent Claim
48 words
The method of any of Claims 12 - 16, comprising a further step of: linking a 5ʹ-untranslated mRNA sequence and a 3ʹ-untranslated mRNA sequence to the gene complex, wherein said 5ʹ-untranslated mRNA and 3ʹ-untranslated mRNA sequence are attached to the 5ʹ- and 3ʹ-ends respectively of said coding sequence.
Claim 24
Dependent Claim
54 words
As a composition of matter, a transgenic mammal for synthesizing biological agents in the mammary gland, having a germ line which includes the recombinant DNA gene complex of any of Claims 1 - 11 or made by the method of any of Claims 12 - 17, said germ line being transmittable to subsequent generations.
Claim 25
Dependent Claim
14 words
A composition of matter according to Claim 18, wherein said transgenic mammal is non-human.
Claim 26
Independent Claim
38 words
A method of targeting the synthesis of biological active agents of at breast one specific gene to the mammary gland, comprising: the step of inserting a recombinant DNA gene complex into a germ line of a mammalian embryo.
Claim 27
Independent Claim
38 words
A method of targeting the synthesis of biological active agents of at breast one specific gene to the mammary gland, comprising: the step of inserting a recombinant DNA gene complex into a germ line of a mammalian embryo.
Claim 28
Dependent Claim
26 words
The method of Claim 20, comprising the further step of: growing said embryo in an environment conducive to differentiate and develop said embryo into a mammal.
Claim 29
Dependent Claim
25 words
The method of Claim 20, comprising the further step of: testing mammary tissue and milk from said mammal for the expression of said coding sequence.
Claim 30
Dependent Claim
22 words
The method of Claim 20, comprising the further step of: confirming the stable incorporation of said gene complex into said germ line.
Claim 31
Dependent Claim
18 words
The method of Claim 20, comprising the further step of: establishing the proper functioning of said gene complex.
Claim 32
Independent Claim
91 words
A method of synthesizing a biologically active agent in mammary gland, comprising of steps of: constructing a recombinant DNA gene complex, said complex comprising a promoter sequence, an enhancer sequence and a signal peptide sequence derived from mammary gland-specific genes and a sequence coding for a biological active agent; inserting said gene complex into a germ line of a mammalian embryo; growing said embryo to maturity; and testing milk produced by said mammal containing the gene complex for said biological active agent and/or recovering the biologically active agent from said milk.
Claim 33
Independent Claim
91 words
A method of synthesizing a biologically active agent in mammary gland, comprising of steps of: constructing a recombinant DNA gene complex, said complex comprising a promoter sequence, an enhancer sequence and a signal peptide sequence derived from mammary gland-specific genes and a sequence coding for a biological active agent; inserting said gene complex into a germ line of a mammalian embryo; growing said embryo to maturity; and testing milk produced by said mammal containing the gene complex for said biological active agent and/or recovering the biologically active agent from said milk.
Claim 34
Independent Claim
25 words
A method of preventing spoilage in milk, comprising the step of: inserting a recombinant DNA gene complex into a germ line of a mammalian embryo.
Claim 35
Independent Claim
25 words
A method of preventing spoilage in milk, comprising the step of: inserting a recombinant DNA gene complex into a germ line of a mammalian embryo.
Claim 36
Dependent Claim
22 words
The method of Claim 26, wherein said gene complex includes a coding sequence derived from a gene coding for a bacteriostatic agent.
Claim 37
Independent Claim
40 words
A method of examining the mechanisms of mammary cancer, comprising the steps of: inserting a recombinant DNA gene complex which includes an oncogene into a germ line of a mammalian embryo; and mechanistically analyzing the resultant development of cancerous tissues.
Claim 38
Independent Claim
40 words
A method of examining the mechanisms of mammary cancer, comprising the steps of: inserting a recombinant DNA gene complex which includes an oncogene into a germ line of a mammalian embryo; and mechanistically analyzing the resultant development of cancerous tissues.
Claim 39
Dependent Claim
33 words
A process for facilitating the production of dairy products, comprising the step of: incorporating customized milk into the production of the dairy products, wherein said customized milk is produced from a transgenic mammal.
Claim 40
Dependent Claim
33 words
A process for facilitating the production of dairy products, comprising the step of: incorporating customized milk into the production of the dairy products, wherein said customized milk is produced from a transgenic mammal.
Claim 41
Dependent Claim
13 words
A food or dairy product, including customized milk produced from a transgenic mammal.
Claim 42
Dependent Claim
13 words
A food or dairy product, including customized milk produced from a transgenic mammal.
Claim 43
Dependent Claim
17 words
A biological active agent, wherein said agent is isolated from customized milk produced from a transgenic mammal.
Claim 44
Dependent Claim
17 words
A biological active agent, wherein said agent is isolated from customized milk produced from a transgenic mammal.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.