EP0278659A2
Expired / Lapsed
EP
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OCS enhancer
Assignee: Agrigenetics Lp En Commonwealth Scientific And
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Filing: 1988-02-02
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Granted: —
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Expires: 2008-02-02
Abstract
Abstract DNA segments which act to increase or activate the expression of genes in plants are described and characterized. These transcription activating elements contain DNA sequences from the upstream untranscribed flanking region of the octopine synthase gene ( ocs ) of the T-DNA of Agrobacterium tumefaciens . The ocs transcription activating element contains a first essential component which has the identifying sequence 5ʹ-ACGTAAGCGTTACGT-3ʹ. A second nonessential component having the identifying sequence 5ʹ-GATGTTAACATC-3ʹ contributes to the level of gene expression. Recombinant DNA molecules containing the plant transcription activating element and a plant-expressible gene under its control are provided. The transcription activating element described and DNA molecules containing it are useful in a method for increasing the level of expression of genes in plant tissue.
Inventors
- 👤 Jeff G. Ellis
- 👤 Daniel J. Llewellyn
- 👤 W. James Peacock
- 👤 Elizabeth Dennis
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 06:28
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/67; C12N15/79; C12N15/82; C12N15/8216
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
41 words
A recombinant DNA molecule comprising a plant transcription activating element capable of activating or enhancing the transcription level of a gene comprising a sequence having about 50% to 100% homology with an identifying sequence selected from 5ʹ-ACGTAAGCGCTTACGT-3ʹ and its reverse sequence.
Claim 2
Dependent Claim
41 words
A recombinant DNA molecule comprising a plant transcription activating element capable of activating or enhancing the transcription level of a gene comprising a sequence having about 50% to 100% homology with an identifying sequence selected from 5ʹ-ACGTAAGCGCTTACGT-3ʹ and its reverse sequence.
Claim 3
Dependent Claim
20 words
A recombinant DNA molecule of claim 1 comprising a sequence having about 75% to 100% homology to said identifying sequence.
Claim 4
Dependent Claim
17 words
A recombinant DNA molecule of claim 1 comprising a sequence having 100% homology to said identifying sequence.
Claim 5
Dependent Claim
41 words
A recombinant DNA molecule comprising the sequence of any of claims 1 to 3 and also comprising a second component comprising a sequence having about 50% to 100% homology with a second identifying sequence selected from 5ʹ-GATGTTAACATC-3ʹ and its reverse sequence.
Claim 6
Dependent Claim
22 words
A recombinant DNA molecule of claim 4 wherein said second component has about 75% to 100% homology to said second identifying sequence.
Claim 7
Dependent Claim
19 words
A recombinant DNA molecule of claim 4 wherein said second component has 100% homology to said second identifying sequence.
Claim 8
Dependent Claim
18 words
The recombinant DNA molecule of any preceding claim wherein said plant transcription activating element is derived from T-DNA.
Claim 9
Dependent Claim
25 words
The recombinant DNA molecule of claim 1 wherein said transcription activating element is derived from the 5ʹ-untranscribed region of the octopine synthase gene of T-DNA.
Claim 10
Dependent Claim
43 words
A recombinant DNA molecule comprising the DNA molecule of any preceding claim in combination with (a) a plant-expressible promoter, and (b) a plant-expressible structural gene wherein said gene is placed under the regulatory control of said transcription activating sequence and said plant-expressible promoter.
Claim 11
Dependent Claim
39 words
The recombinant DNA molecule of claim 9 wherein the transcription activating element is located between the 5ʹ end of the TATA box of said promoter and about 1500 bp 5ʹ of the transcription initiation site of said plant-expressible gene.
Claim 12
Dependent Claim
70 words
The recombinant DNA molecule of claim 9 wherein said transcription activating element comprises a second component, consisting essentially of a sequence having about 50% to 100% homology with a second identifying sequence selected from 5ʹ-GATGTTAACATC-3ʹ and its reverse sequence located 5ʹ to said plant-expressible promoter, and wherein said second component is located between about 1 and about 500 bp of the identifying sequence of said DNA molecule of claim 1.
Claim 13
Dependent Claim
19 words
The recombinant DNA molecule of any of claims 9 to 11 wherein both said elements are derived from T-DNA.
Claim 14
Dependent Claim
18 words
The recombinant DNA molecule of claim 12 wherein both said elements are derived from an octopine synthase gene.
Claim 15
Dependent Claim
22 words
The recombinant DNA molecule of claim 9 wherein said transcription activating element is located 3ʹ to said promoter and said structural gene.
Claim 16
Independent Claim
68 words
A method for enhancing the expression of a plant-expressible gene in plant tissue comprising the steps of (a) inserting a transcription activating element comprising a sequence having about 50% to 100% homology to an identifying sequence selected from 5ʹ-ACGTAAGCGCTTACGT-3ʹ and its reverse sequence, in such a way that said transcription activating element modulates the expression of said gene, and (b) introducing said recombinant DNA molecule into plant tissue.
Claim 17
Independent Claim
68 words
A method for enhancing the expression of a plant-expressible gene in plant tissue comprising the steps of (a) inserting a transcription activating element comprising a sequence having about 50% to 100% homology to an identifying sequence selected from 5ʹ-ACGTAAGCGCTTACGT-3ʹ and its reverse sequence, in such a way that said transcription activating element modulates the expression of said gene, and (b) introducing said recombinant DNA molecule into plant tissue.
Claim 18
Dependent Claim
14 words
The method of claim 15 wherein said plant tissue is from a monocotyledonous plant.
Claim 19
Dependent Claim
14 words
The method of claim 15 wherein said plant tissue is from a dicotyledonous plant.
Claim 20
Independent Claim
22 words
The method of any of claims 15 to 17 wherein said recombinant DNA molecule is introduced into said plant tissue by electroporation.
Claim 21
Independent Claim
34 words
The method of any of claims 15 to 18 wherein said transcription activating element also comprises a sequence having about 50% to 100% homology to a sequence selected from 5ʹ-GATGTTAACATC-3ʹ and its reverse sequence.
References Cited (Backward Citations)
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