EP0243151A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Modified microbially-produced cellulose gel and complex thereof with animal cell
Assignee: Ajinomoto Co Inc
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Filing: 1987-04-22
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Granted: —
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Expires: 2007-04-22
Abstract
Abstract Disclosed is a gel of microbially-produced cellulose, characterized in that the microbially-produced cellulose is modified by (l) physically or chemically bonding an animal cell adhesive protein to the cellulose. and/or (2) substituting hydrogen atoms of at least some hydroxyl groups of the cellulose with a positively or negatively charged organic group. This gel is valuable as a carrier for mass culture of animal cells or as a medical vulnerary cover.
Inventors
- 👤 Shigeru C/O Central Research Lab. Yamanaka
- 👤 Yuzuru C/O Central Research Lab. Eto
- 👤 Satoshi C/O Central Research Lab. Takano
- 👤 Kunihiko C/O Central Research Lab. Watanabe
- 👤 Hiroshiro C/O Central Research Lab. Shibai
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 07:28
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/50; A61L27/60; C; C08; C08B; C08B11/00; C08B11/02; C08B11/04; C08B11/14; C08B11/145; C08L; C08L1/00; C08L1/02; C12; C12N; C12N5/00; C12N5/0068; C12N2533/00; C12N2533/70; C12N2533/78; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
84 words
A gel of microbially-produced cellulose, characterized in that the microbially-produced cellulose is modified by (1) physically or chemically bonding an animal cell adhesive protein to the cellulose; (2) substituting hydrogen atoms of at least some hydroxyl groups of the cellulose with a positively or negatively charged organic group; or (3) substituting hydrogen atoms of at least some hydroxyl groups of the cellulose with a positively or negatively charged organic group and further physically or chemically bonding an animal cell adhesive protein to the cellulose.
Claim 2
Dependent Claim
84 words
A gel of microbially-produced cellulose, characterized in that the microbially-produced cellulose is modified by (1) physically or chemically bonding an animal cell adhesive protein to the cellulose; (2) substituting hydrogen atoms of at least some hydroxyl groups of the cellulose with a positively or negatively charged organic group; or (3) substituting hydrogen atoms of at least some hydroxyl groups of the cellulose with a positively or negatively charged organic group and further physically or chemically bonding an animal cell adhesive protein to the cellulose.
Claim 3
Dependent Claim
77 words
A gel of microbially-produced cellulose as set forth in claim 1, wherein the positively charged organic group is represented by the following formula (I) or (II): wherein n is an integer of from 0 to 8, and R₁ , R₂ and R₃ independently represent a hydrogen atom or an alkyl, aryl, aralkyl, alkaryl, cycloalkyl or alkoxyalkyl group, with the proviso that the case where all of R₁ , R₂ and R₃ represent a hydrogen atom is excluded.
Claim 4
Dependent Claim
35 words
A gel of microbially-produced cellulose as set forth in claim 1, wherein the negatively charged organic group is selected from a carboxymethyl group, a carboxymethyl group, a phosphoric acid group and a sulfuric acid group.
Claim 5
Dependent Claim
30 words
A gel of microbially-produced cellulose as set forth in claim 1 or claim 2 or 3, wherein the animal cell adhesive protein is selected from collagen, fibronectin, laminin and gelatin.
Claim 6
Dependent Claim
24 words
A gel of microbially-produced cellulose as set forth in claim 1 or 4,wherein the chemical bonding is crosslinking through an epihalohydrin or cyanogen halide.
Claim 7
Dependent Claim
35 words
A gel of microbially-produced cellulose as set forth in any of claims l to 5, wherein at least one member selected from enzymes, bacteriostats, antibacterial agents, chemotherapeutants, coagulants and anticoagulants is chemically or physically bonded.
Claim 8
Dependent Claim
22 words
A gel of microbially-produced cellulose as set forth in any of claims l to 6, which is complexed with an auxiliary material.
Claim 9
Dependent Claim
41 words
A complex of a gel of microbially-produced cellulose with an animal cell bonded to or adsorbed in said gel of the microbially-produced cellulose, characterized in that the microbially-produced cellulose is a modified microbially produced cellulose as claimed in any preceding claim.
Claim 10
Dependent Claim
21 words
A complex as set forth in claim 8, wherein the bonded or adsorbed animal cell is an anchorage dependent animal cell.
Claim 11
Dependent Claim
19 words
A complex as set forth in claim 9, wherein the anchorage dependent animal cell is a human epidermal cell.
Claim 12
Dependent Claim
27 words
A complex as set forth in claim 10, wherein the human epidemic cell is cultured substantially in the monolayer state on the gel of the microbially-produced cellulose.
Claim 13
Dependent Claim
21 words
A complex as set forth in claim 8, wherein the bonded or adsorbed animal cell is an anchorage independent animal cell.
Claim 14
Dependent Claim
20 words
A complex of a gel of a microbially-produced cellulose with an animal cell bonded to or adsorbed in said gel.
Claim 15
Dependent Claim
20 words
A complex of a gel of a microbially-produced cellulose with an animal cell bonded to or adsorbed in said gel.
Claim 16
Dependent Claim
28 words
A complex as set forth in claim 13, wherein at least one member selected from enzymes, bacteriostats, antibacterial agents, chemotherapeutants, coagulants and anticoagulants is chemically or physically bonded.
Claim 17
Dependent Claim
22 words
A complex as set forth in claim 13 or 14, wherein the microbially-produced cellulose gel is further complexed with an auxiliary material.
Claim 18
Dependent Claim
25 words
A complex as set forth in any of claims 13 to 15, wherein the bonded or adsorbed animal cell is an anchorage dependent animal cell.
Claim 19
Dependent Claim
19 words
A complex as set forth in claim 16, wherein the anchorage adherent animal cell is a human epidermal cell.
Claim 20
Dependent Claim
27 words
A complex as set forth in claim 17, wherein the human epidemic cell is cultured substantially in the monolayer state on the gel of the microbially-produced cellulose.
Claim 21
Dependent Claim
25 words
A complex as set forth in any of claims 13 to 15, wherein the bonded or adsorbed animal cell is an anchorage independent animal cell.
References Cited (Backward Citations)
No citation records indexed.
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