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EP0243132A2 Expired / Lapsed EP ⏱️ 0.0 years remaining

Artificial skin and its production

Assignee: Koken Co Ltd
•
Filing: 1987-04-16
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Granted: —
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Expires: 2007-04-16
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Abstract

Abstract An artificial skin comprising an insoluble atelocollagen sheet and an epidermal cell layer cultured only on one surface of the sheet, and a method of producing the same are disclosed, which method includes the steps of: forming an insoluble atelocollagen sheet; inoculating epidermal cells only on one surface of the atelocollagen sheet; and suspending the atelocollagen sheet in a liquid culture medium to culture the epidermal cells, thereby forming an epidermal cell layer.

Inventors

  • 👤 Yoshizato Katsutoshi
  • 👤 Taira Toshio
  • 👤 Miyata Teruo

Bibliographic Details

Application Number —
Jurisdiction EP
Data Source google_patents
Last Synchronized 2026-10-23 14:04
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/14; A61L27/22; A61L27/24; A61F; A61F2/00; A61F2/02; A61F2/10; A61F2/105; A61L27/50; A61L27/60; A61F2310/00; A61F2310/00005; A61F2310/00365; Y; Y10; Y10S; Y10S623/00; Y10S623/901

Claims Analysis

Total Claims: 7
Claim 1
Independent Claim 20 words
An artifical skin comprising an insoluble atelocollagen sheet and an epidermal cell layer cultured on one surface of said sheet.
Claim 2
Dependent Claim 20 words
An artifical skin comprising an insoluble atelocollagen sheet and an epidermal cell layer cultured on one surface of said sheet.
Claim 3
Dependent Claim 31 words
An artificial skin according to claim 1 wherein said atelocollagen sheet has a fibroblast layer cultured on at least one surface thereof and an epidermal cell layer on said fibroblast layer.
Claim 4
Dependent Claim 28 words
An artificial skin according to claim 1 wherein said atelocollagen sheet has a fibroblast layer cultured on the opposite surface thereof to that having said epidermal cell layer.
Claim 5
Independent Claim 40 words
A method of producing an artificial skin comprising the steps of: forming an insoluble atelocollagen sheet; inoculating epidermal cells on one surface of said atelocollagen sheet; and culturing the epidermal cells on the sheet to form an epidermal cell layer.
Claim 6
Independent Claim 40 words
A method of producing an artificial skin comprising the steps of: forming an insoluble atelocollagen sheet; inoculating epidermal cells on one surface of said atelocollagen sheet; and culturing the epidermal cells on the sheet to form an epidermal cell layer.
Claim 7
Dependent Claim 31 words
A method according to claim 4, wherein fibroblasts are cultured on at least one surface of said atelocollagen sheet to form a fibroblast layer, and thereafter the epidermal cells are inoculated.

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