EP0228225A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Immunoassay kit and method employing modified solid surface
Assignee: Ortho Clinical Diagnostics Inc
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Filing: 1986-12-15
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Granted: —
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Expires: 2006-12-15
Abstract
Abstract An immunoassay kit and method is described. The kit includes a solid surface having immobilized thereon a biologically specific substance for detecting the presence of an analyte. The substance is covalently bonded to the solid surface by a difunctional hydrophilic group having a molecular chain length of about 10 to about 300 Angstroms.
Inventors
- 👤 Amy L. Finkenaur
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 08:15
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54353
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
52 words
An immunoassay kit including a solid surface having immobilized thereon a biologically specific substance for detecting the presence of a predetermined biological analyte, wherein said biologically specific substance is covalently bonded to said surface by a difunctional hydrophilic group having a molecular chain length of from about 10 to about 300 Angstroms.
Claim 2
Dependent Claim
52 words
An immunoassay kit including a solid surface having immobilized thereon a biologically specific substance for detecting the presence of a predetermined biological analyte, wherein said biologically specific substance is covalently bonded to said surface by a difunctional hydrophilic group having a molecular chain length of from about 10 to about 300 Angstroms.
Claim 3
Dependent Claim
15 words
The immunoassay kit of claim 1 wherein said difunctional hydrophilic group comprises a polyoxyethylene group.
Claim 4
Dependent Claim
36 words
The immunoassay kit of claim 1 or claim 2 wherein said biologically specific substance is a peptide, protein, cell wall material, viral particle, enzyme, bacterial antigen, a nucleic acid, an antibody, DNA, RNA, or a carbohydrate.
Claim 5
Dependent Claim
35 words
The immunoassay kit of any one of claims 1 to 3 wherein said biologically specific substance is bonded to said difunctional hydrophilic group by an amide group, a disulphide group or a secondary amino group.
Claim 6
Dependent Claim
19 words
The immunoassay kit of any one of claims1 to 4 wherein said solid surface comprises a styrene/maleic anhydride copolymer.
Claim 7
Dependent Claim
22 words
The immunoassay kit of claim 5 wherein said difunctional hydrophilic group is bonded to said solid surface by a secondary amino group.
Claim 8
Dependent Claim
20 words
The immunoassay kit of any one of claims 1 to 4 wherein said solid surface comprises a styrene/maleic anhydride copolymer.
Claim 9
Independent Claim
111 words
A method for detecting for the presence of a predetermined biological analyte which comprises the steps of: a) incubating a material to be analyzed with a solid surface having immobilized thereon a first biologically specific substance that will selectively bind to said analyte, said first biologically specific substance being covalently bonded to said surface by a difunctional hydrophilic group having a molecular chain length of from about 10 to about 300 Angstroms; b) incubating said surface with a labelled biologically specific substance that will bind only to said analyte; c) washing said surface after step b); and d) analyzing for the presence of the label on said labelled biologically specific substance.
Claim 10
Independent Claim
111 words
A method for detecting for the presence of a predetermined biological analyte which comprises the steps of: a) incubating a material to be analyzed with a solid surface having immobilized thereon a first biologically specific substance that will selectively bind to said analyte, said first biologically specific substance being covalently bonded to said surface by a difunctional hydrophilic group having a molecular chain length of from about 10 to about 300 Angstroms; b) incubating said surface with a labelled biologically specific substance that will bind only to said analyte; c) washing said surface after step b); and d) analyzing for the presence of the label on said labelled biologically specific substance.
Claim 11
Dependent Claim
20 words
The process of claim 8 wherein said process comprises an Enzyme-Linked Immunosorbent Assay, a Radioimmune Assay, or an agglutination assay.
Claim 12
Dependent Claim
16 words
The process of claim 8 or claim 9 wherein said hydrophilic group comprises a polyoxyethylene group.
References Cited (Backward Citations)
No citation records indexed.
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