Abstract Polypeptide capable of differentiating and maturating mouse leukemia cells into normal cells, having the following properties : (a) Molecular weight 16±1 Kd ( SDS-electrophoresis in the presence of 1.0% mercaptoethanol ) 25±1 Kd ( SDS-electrophoresis in the absence of mercaptoethanol ) (b) Amino acid sequence at amino terminal Gly-Leu-Glu-X-Asp-Gly-Lys-Val-Asn-Ile-X-X-Lys-Lys-Gln-Phe-Phe-Val-Ser-Phe-Lys-Asp-Ile-Gly-Trp-Asn-Asp-Trp-Ile-Ile-Ala-Pro-Ser-Gly-Tyr ( X: unidentified amino acid ) (c) Amino acid sequence of peptide fragment formed by cyanogen bromide cleavage Leu-Tyr-Tyr-Asp-Asp-Gly-Gln-Asn-Ile-Ile-Lys-Lys-Asp-Ile-Gln-Asn
A polypeptide capable of differentiating and maturating mouse leukemia cells into normal cells and having the following properties: (a) Molecular weight 16±1 Kd ( SDS-electrophoresis in the presence of 1.0% mercaptoethanol ) 25±1 Kd ( SDS-electrophoresis in the absence of mercaptoethanol ) (b) Amino acid sequence at amino terminal Gly-Leu-Glu-X-Asp-Gly-Lys-Val-Asn-Ile-X-X-Lys-Lys-Gln-Phe-Phe-Val-Ser-Phe-Lys-Asp-Ile-Gly-Trp-Asn-Asp-Trp-Ile-Ile-Ala-Pro-Ser-Gly-Tyr ( X: unidentified amino acid ) (c) Amino acid sequence of peptide fragment formed by cyanogen bromide cleavage Leu-Tyr-Tyr-Asp-Asp-Gly-Gln-Asn-Ile-Ile-Lys-Lys-Asp-Ile-Gln-Asn
Claim 2
Dependent Claim
69 words
A polypeptide capable of differentiating and maturating mouse leukemia cells into normal cells and having the following properties: (a) Molecular weight 16±1 Kd ( SDS-electrophoresis in the presence of 1.0% mercaptoethanol ) 25±1 Kd ( SDS-electrophoresis in the absence of mercaptoethanol ) (b) Amino acid sequence at amino terminal Gly-Leu-Glu-X-Asp-Gly-Lys-Val-Asn-Ile-X-X-Lys-Lys-Gln-Phe-Phe-Val-Ser-Phe-Lys-Asp-Ile-Gly-Trp-Asn-Asp-Trp-Ile-Ile-Ala-Pro-Ser-Gly-Tyr ( X: unidentified amino acid ) (c) Amino acid sequence of peptide fragment formed by cyanogen bromide cleavage Leu-Tyr-Tyr-Asp-Asp-Gly-Gln-Asn-Ile-Ile-Lys-Lys-Asp-Ile-Gln-Asn
Claim 3
Dependent Claim
38 words
A polypeptide capable of differentiating and maturating mouse leukemia cells into normal cells, obtainable by culturing human leukemia cells in the presence of at least one differentiation inducing substance and isolating the accumulated polypeptide from the culture medium.
Claim 4
Dependent Claim
38 words
A polypeptide capable of differentiating and maturating mouse leukemia cells into normal cells, obtainable by culturing human leukemia cells in the presence of at least one differentiation inducing substance and isolating the accumulated polypeptide from the culture medium.
Claim 5
Dependent Claim
22 words
A polypeptide according to claim 2, wherein the differentiation inducing substance is Actinomycin D, Mitomycin C, concanavalin A or a phorbol ester.
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