Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
28 words
A process for stabilizing and flocculating intact or ruptured microbial cells having active enzymes associated therewith which comprises contacting the microbial cells with a partially modified cationic polyelectrolyte.
Claim 2
Dependent Claim
28 words
A process for stabilizing and flocculating intact or ruptured microbial cells having active enzymes associated therewith which comprises contacting the microbial cells with a partially modified cationic polyelectrolyte.
Claim 3
Dependent Claim
25 words
Process of Claim 1 which comprises (1) encapsulating the flocculated microbial cells with a partially modified cationic polyelectrolyte, and (2) cross-linking the encapsulated microbial cells.
Claim 4
Dependent Claim
16 words
Process of Claim 2 wherein the flocculated microbial cells are cross-linked both before and after encapsulating.
Claim 5
Dependent Claim
18 words
The process of any one of Claims 1 to 3 wherein the microbial cells are glucose isomerase--producing cells.
Claim 6
Dependent Claim
21 words
The process of any one of Claims 1 to 4 wherein the partially modified cationic polyelectrolyte is a partially modified polyamine.
Claim 7
Dependent Claim
52 words
The process of any one of Claims 1 to 5 wherein the partially modified cationic polyelectrolyte is a partially carboxyalkylated, or partially phosphonoalkylated, cationic polyelectrolyte wherein the alkyl is -(CH 2 ) n - wherein n=1-3, or -(CHR)-(CH 2 ) n - wherein R=methyl, ethyl, propyl, or isopropyl, and n=l or 2.
Claim 8
Dependent Claim
15 words
The process of Claim 6 wherein the partially carboxyalkylated cationic polyelectrolyte is partially carboxymethylated polyethyleneimine.
Claim 9
Dependent Claim
42 words
The process of any one of Claims 1 to 7 wherein the modification of the partially modified cationic polyelectrolyte is from 0.1 to 1.0 equivalents of polyelectrolyte during the flocculating step and 0.2 to 1.0 equivalents of polyelectrolyte during the encapsulating steps.
Claim 10
Dependent Claim
27 words
The process of Claim 8 wherein the partially modified cationic polyelectrolyte is polyethyleneimine which is carboxymethylated in the range of 0.25 to 0.5 equivalents of the imine.
Claim 11
Dependent Claim
33 words
A process for preparing D-fructose from D-glucose which comprises contacting D-glucose with glucose isomerase-producing intact or ruptured microbial cells which have been 1) flocculated with a partially modified cationic polyelectrolyte, and (2) cross-linked.
Claim 12
Dependent Claim
33 words
A process for preparing D-fructose from D-glucose which comprises contacting D-glucose with glucose isomerase-producing intact or ruptured microbial cells which have been 1) flocculated with a partially modified cationic polyelectrolyte, and (2) cross-linked.
Claim 13
Dependent Claim
21 words
Process for Claim 10 wherein the microbial cells employed have been encapsulated with a partially modified cationic polyelectrolyte, and agëin cross-linked.