EP0174835A1
Expired / Lapsed
EP
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Human tissue plasminogen activator and recombinant DNA compounds
Assignee: Pharmacia and Upjohn Co
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Filing: 1985-09-10
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Granted: —
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Expires: 2005-09-10
Abstract
Abstract A human tissue plasminogen activator, and analogues thereof, which are the product of expression of recombinant DNA in yeast. The products are useful as fibrinolytic agents.
Inventors
- 👤 Gurnam S. Gill
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 16:28
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; C12N9/6459; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/81; C12Y; C12Y304/00; C12Y304/21; C12Y304/21069; C07; C07K; C07K2319/00; C07K2319/01; C07K2319/02
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
4 words
t-PA having selected glycosylation.
Claim 2
Dependent Claim
4 words
t-PA having selected glycosylation.
Claim 3
Dependent Claim
5 words
t-PA when expressed by yeast.
Claim 4
Dependent Claim
5 words
t-PA when expressed by yeast.
Claim 5
Dependent Claim
12 words
A t-PA according to Claim 2 wherein the yeast is Saccharomyces cerevisiae.
Claim 6
Dependent Claim
113 words
A circular recombinant DNA compound of formula XXX wherein the repeated alphabetic sequences (the number of repetitions of which is arbitrarily depicted in said formula) therein represent the following: the "P" region comprises nucleotides whose sequence contains a yeast promoter and "pre-pro" signals consisting essentially of the "pre-pro" signals from the MFa1 gene; the "T" region comprises nucleotides whose sequence contains DNA for the synthesis of t-PA; the "H" region comprises the nucleotide whose sequence contains the 3'-end of the ADHI gene; the "A" region comprises the nucleotides whose ordered codons contain the URA3 gene; the "R" region comprises nucleotides whose sequence contains a REPIII and replication origin from the 2p yeast plasmid.
Claim 7
Dependent Claim
113 words
A circular recombinant DNA compound of formula XXX wherein the repeated alphabetic sequences (the number of repetitions of which is arbitrarily depicted in said formula) therein represent the following: the "P" region comprises nucleotides whose sequence contains a yeast promoter and "pre-pro" signals consisting essentially of the "pre-pro" signals from the MFa1 gene; the "T" region comprises nucleotides whose sequence contains DNA for the synthesis of t-PA; the "H" region comprises the nucleotide whose sequence contains the 3'-end of the ADHI gene; the "A" region comprises the nucleotides whose ordered codons contain the URA3 gene; the "R" region comprises nucleotides whose sequence contains a REPIII and replication origin from the 2p yeast plasmid.
Claim 8
Dependent Claim
24 words
A compound of Claim 4 wherein the "T" region does not contain the 3'-end untranslated region of the DNA for the synthesis of t-PA.
Claim 9
Dependent Claim
17 words
A yeast cell or cell line whose expressed genetic material comprises a DNA compound of Claim 4.
Claim 10
Dependent Claim
18 words
A cell or cell line according to Claim 8 wherein the DNA compound of Claim 4 expresses t-PA.
Claim 11
Dependent Claim
15 words
A method of producting t-PA which comprises culturing the cell line according to Claim 7.
References Cited (Backward Citations)
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