EP0173339A2
Expired / Lapsed
EP
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Composition and method for the dectection of the presence of a polynucleotide sequence of interest
Assignee: Enzo Biochem Inc
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Filing: 1985-08-29
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Granted: —
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Expires: 2005-08-29
Abstract
Abstract Described is a genetic analyses using the technique of nucleic acid hybridization. The genetic analyses includes, for example, the diagnosis of infections by foreign microbes and the detection of specific genetic traits and abnormalities. Furthermore, a technique for the detection of the presence of a polynucleotide sequence of interest is described.
Inventors
- 👤 Elazar Rabbani
- 👤 Dean L. Engelhardt
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 19:35
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/70; C12Q1/68; C12Q1/6813; C12Q1/6832; C12Q1/6876; C12Q1/6888; C12Q1/689; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
119 words
A composition for detecting a polynucleotide sequence of interest in a sample which may contain polynucleotide sequences not of interest which comprises: (a) a first polynucleotide sequence wherein said first polynucleotide sequence is substantially complementary to and capable of hybridizing to said polynucleotide sequence of interest and is labeled with a first detectable marker; (b) a second polynucleotide sequence wherein said second polynucleotide sequence is not substantially complementary to or substantially identical to said polynucleotide sequence of interest and is labeled with said first detectable marker; and (c) a third polynucleotide sequence wherein said third polynucleotide sequence is substantially complementary to or identical to said second polynucleotide sequence and is either unlabeled or labeled with a second detectable marker.
Claim 2
Dependent Claim
119 words
A composition for detecting a polynucleotide sequence of interest in a sample which may contain polynucleotide sequences not of interest which comprises: (a) a first polynucleotide sequence wherein said first polynucleotide sequence is substantially complementary to and capable of hybridizing to said polynucleotide sequence of interest and is labeled with a first detectable marker; (b) a second polynucleotide sequence wherein said second polynucleotide sequence is not substantially complementary to or substantially identical to said polynucleotide sequence of interest and is labeled with said first detectable marker; and (c) a third polynucleotide sequence wherein said third polynucleotide sequence is substantially complementary to or identical to said second polynucleotide sequence and is either unlabeled or labeled with a second detectable marker.
Claim 3
Dependent Claim
32 words
The composition according to Claim 1, wherein said first polynucleotide sequence and said second polynucleotide sequence are derived from a recombinant molecule wherein said second polynucleotide sequence comprises a vector polynucleotide sequence.
Claim 4
Dependent Claim
22 words
The composition according to Claim 1, wherein said first polynucleotide sequence is covalently linked to said second polynucleotide sequence in a chromosome.
Claim 5
Dependent Claim
49 words
The composition according to Claim 3 wherein said first polynucleotide sequence is specific for a polynucleotide sequence selected from N . gonorrhoea, herpes simplex virus I, herpes simplex virus II, Brucella abortus, Bordetella pertussis, Shigella dysenteria, Haemophilus influenzae, Mycobacterium tuberculosis, Pseudomonas pseudomallei, Salmonella typhi, Salmonella typhimurium or N. meningitidis.
Claim 6
Dependent Claim
16 words
The composition according to Claim 1, wherein said second polynucleotide sequence is a host polynucleotide sequence.
Claim 7
Dependent Claim
12 words
The composition according Claim 1, wherein said third polynucleotide sequence is unlabeled.
Claim 8
Independent Claim
204 words
A method for the detection of a polynucleotide sequence of interest in the potential or actual presence of polynucleotide sequences not of interest which comprises: (A) providing a composition which comprises: (i) a first polynucleotide sequence wherein said first polynucleotide sequence is substantially complementary to and capable of hybridizing to said polynucleotide sequence of interest and is labeled with a first detectable marker; (ii) a second polynucleotide sequence wherein said second polynucleotide sequence is not substantially complementary to or substantially identical to said polynucleotide sequence of interest and is labeled with said first detectable marker; (iii) a third polynucleotide sequence wherein said third polynucleotide sequence is substantially complementary to or identical to said second polynucleotide sequence and is either unlabeled or labeled with a second detectable marker; (B) rendering at least a portion of said polynucleotide sequence of interest and said polynucleotide sequences not of interest in single stranded form; (C) rendering at least a portion of said . composition in single stranded form; (D) contacting said polynucleotide sequence of interest and said polynucleotide sequences not of interest in said sample with said composition under conditions to permit hybridization; and (E) detecting said polynucleotide sequence of interest by means of said first detectable marker.
Claim 9
Independent Claim
204 words
A method for the detection of a polynucleotide sequence of interest in the potential or actual presence of polynucleotide sequences not of interest which comprises: (A) providing a composition which comprises: (i) a first polynucleotide sequence wherein said first polynucleotide sequence is substantially complementary to and capable of hybridizing to said polynucleotide sequence of interest and is labeled with a first detectable marker; (ii) a second polynucleotide sequence wherein said second polynucleotide sequence is not substantially complementary to or substantially identical to said polynucleotide sequence of interest and is labeled with said first detectable marker; (iii) a third polynucleotide sequence wherein said third polynucleotide sequence is substantially complementary to or identical to said second polynucleotide sequence and is either unlabeled or labeled with a second detectable marker; (B) rendering at least a portion of said polynucleotide sequence of interest and said polynucleotide sequences not of interest in single stranded form; (C) rendering at least a portion of said . composition in single stranded form; (D) contacting said polynucleotide sequence of interest and said polynucleotide sequences not of interest in said sample with said composition under conditions to permit hybridization; and (E) detecting said polynucleotide sequence of interest by means of said first detectable marker.
Claim 10
Dependent Claim
40 words
The method according to Claim 7, wherein substantially all of said polynucleotide sequence of interest and said polynucleotide sequences not of interest are rendered in single stranded form and substantially all of said composition is rendered in single stranded form.
Claim 11
Dependent Claim
56 words
The method according to Claim 8, wherein said polynucleotide sequence of interest and said polynucleotide sequences not of interest in said sample are contacted with said third polynucleotide sequence prior to contacting polynucleotide sequence of interest and said polynucleotide sequences not of interest in said sample with said first polynucleotide sequence and said second polynucleotide sequence.
Claim 12
Dependent Claim
53 words
The method according to Claim 8, which further comprises contacting said second polynucleotide sequence with said third polynucleotide sequence under conditions to permit hybridization after said composition is rendered into substantially single stranded form, but before said polynucleotide sequence of interest and said polynucleotide sequences not of interest are contacted with said composition.
References Cited (Backward Citations)
No citation records indexed.
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