EP0161640A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Encapsulated mouse cells transformed with avian retrovirus-bovine growth hormone DNA, and a method of administering BGH in vivo
Assignee: Merck and Co Inc
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Filing: 1985-05-10
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Granted: —
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Expires: 2005-05-10
Abstract
Abstract Recombinant DNA constructs having an avian retroviral long terminal repeat (LTR) ligated to the bovine growth hormone gene, were co-transformed into a mammalian cell (mouse) culture in order to obtain a stable cell culture secreting large amounts of bovine growth hormone. The transformed mouse cells were encapsulated in hollow fibers and implanted into animals, thereby producing circulating bovine growth hormone.
Inventors
- 👤 John J. Kopchick
- 👤 Frederick C. Leung
- 👤 Thomas J. Livelli
- 👤 Richard H. Malavarca
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 10:56
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/0087; A61K9/0092; A01; A01K; A01K67/00; A01K67/027; A01K67/0271; A61K38/00; A61K38/16; A61K38/17; A61K38/22; A61K38/27; A61K9/0012; A61K9/0019; A61K9/0024; C; C07; C07K; C07K14/00; C07K14/435; C07K14/575; C07K14/61; C12; C12N; C12N11/00; C12N11/02; C12N11/04; A61K48/00
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
32 words
The method for increasing milk production in cows by in vivo implantation in said cows of an effective amount of the encapsulated cell line L-BGH-4-3, ATCC CRL 8537 or L-BGH-4-13, ATCC CRL-8536.
Claim 2
Independent Claim
32 words
The method for increasing milk production in cows by in vivo implantation in said cows of an effective amount of the encapsulated cell line L-BGH-4-3, ATCC CRL 8537 or L-BGH-4-13, ATCC CRL-8536.
Claim 3
Independent Claim
29 words
The method for increasing animal growth by implantation in vivo in said animals an effective amount of the encapsulated cell line L-BGH-4-3, ATCC CRL 8537 or L-BGH-4-13, ATCC CRL-8536.
Claim 4
Independent Claim
29 words
The method for increasing animal growth by implantation in vivo in said animals an effective amount of the encapsulated cell line L-BGH-4-3, ATCC CRL 8537 or L-BGH-4-13, ATCC CRL-8536.
Claim 5
Dependent Claim
16 words
The methods of Claims 1 or 2 in which the encapsulated cell line is implanted subcutaneously.
Claim 6
Dependent Claim
45 words
A hollow fiber, about 10 mm long polyvinyl chloride-acrylic copolymer, having about a 1100 µm internal diameter, containing a recombinant mouse cell suspension capable of producing bovine growth hormone in an amount sufficient to stimulate animal growth or milk production when implanted in an animal.
Claim 7
Dependent Claim
45 words
A hollow fiber, about 10 mm long polyvinyl chloride-acrylic copolymer, having about a 1100 µm internal diameter, containing a recombinant mouse cell suspension capable of producing bovine growth hormone in an amount sufficient to stimulate animal growth or milk production when implanted in an animal.
Claim 8
Dependent Claim
64 words
A hollow fiber, about 10 mm long, polyvinylchloride-acrylic copolymer, having about a 1100 U m internal diameter, containing a cell suspension which has been previously transfected with biologically active DNA, said cells having been selected for production and secretion of the corresponding protein product, the cell suspension thusly being capable of producing the protein in a biologically active amount when implanted in an animal.
Claim 9
Dependent Claim
64 words
A hollow fiber, about 10 mm long, polyvinylchloride-acrylic copolymer, having about a 1100 U m internal diameter, containing a cell suspension which has been previously transfected with biologically active DNA, said cells having been selected for production and secretion of the corresponding protein product, the cell suspension thusly being capable of producing the protein in a biologically active amount when implanted in an animal.
References Cited (Backward Citations)
No citation records indexed.
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