Antibodies to human interleukin-2 induced by synthetic polypeptides

Claim 2

A synthetic polypeptide containing about 6 to about 40 amino acid residues having an amino acid residue sequence that substantially corresponds to an amino acid residue sequence of an antigenic determinant of interleukin-2, said synthetic polypeptide having the capacity alone, as a polymer or as a conjugate of said polypeptide bound to a carrier, when injected into a host in an effective amount and in a physiologically tolerable diluent of inducing the production of antibodies to said antigenic determinant of interleukin-2.

Claim 3

The synthetic polypeptide according to claim 1 including the sequence of amino acid residues, taken from left to right and in the direction from amino-terminus to carboxy-terminus, represented by the formula: ThrAsnSerAlaProThrSerSerSerThrLysLysThrGlnLeu.

Claim 4

The synthetic polypeptide according to claim 1 including the sequence of amino acid residues, taken from left to right and in the direction from amino-terminus to carboxy-terminus, represented by the formula: LeuGluGluGluLeuLysProLeuGluGluValLeuAsnLeu.

Claim 5

The synthetic polypeptide according to claim 1 including the sequence of amino acid residues, taken from left to right and in the direction from amino-terminus to carboxy-terminus, represented by-the formula: ValIleValLeuGluLeuLysGlySerGluThrThrPheMetCys.

Claim 6

The synthetic polypeptide according to claim 1 including the sequence of amino acid residues, taken from left to right and in the direction from amino-terminus to carboxy-terminus, represented by the formula: AsnArgTrpIleThrPheCysGlnSerIleIleSerThrLeuThr.

Claim 7

An inoculum comprising an effective amount of a synthetic polypeptide containing about 6 to about 40 amino acid residues having an amino acid residue sequence that immunologically corresponds substantially to an amino acid sequence of an antigenic determinant of interleukin-2 and a physiologically tolerable diluent, said inoculum, when introduced into a host, being capable of inducing the production of antibodies in the host that immunoreact with said antigenic determinant of interleukin-2.

Claim 8

An inoculum comprising an effective amount of a synthetic polypeptide containing about 6 to about 40 amino acid residues having an amino acid residue sequence that immunologically corresponds substantially to an amino acid sequence of an antigenic determinant of interleukin-2 and a physiologically tolerable diluent, said inoculum, when introduced into a host, being capable of inducing the production of antibodies in the host that immunoreact with said antigenic determinant of interleukin-2.

Claim 9

The inoculum according to claim 6 wherein said synthetic polypeptide includes the sequence of amino acid residues, taken from left to right and in the direction from amino-terminus to carboxy-terminus, represented by the formula: ThrAsnSerAlaProThrSerSerSerThrLysLysThrGlnLeu.

Claim 10

The inoculum according to claim 6 wherein said synthetic polypeptide includes the sequence of amino acid residues, taken from left to right and in the direction from amino-terminus to carboxy-terminus, represented by the formula: LeuGluGluGluLeuLysProLeuGluGluValLeuAsnLeu.

Claim 11

The inoculum according to claim 6 wherein said synthetic polypeptide includes the sequence of amino acid residues, taken from left to right and in the direction from amino-terminus to carboxy-terminus, represented by the formula: ValIleValLeuGluLeuLysGlySerGluThrThrPheMetCys.

Claim 12

The inoculum according to claim 6 wherein said synthetic polypeptide includes the sequence of amino acid residues, taken from left to right and in the direction from amino-terminus to carboxy-terminus, represented by the formula: AsnArgTrpIleThrPheCysGlnSerIleIleSerThrLeuThr.

Claim 13

The inoculum according to claim 6 wherein said synthetic polypeptide is bound to a carrier is selected from the group consisting of keyhole limpet hemocyanin, keyhole limpet hemocyanin in incomplete Freund's adjuvant, alum, keyhole limpet hemocyanin-alum absorbed, keyhole limpet hemocyanin-alum absorbed-pertussis, edestin, thyroglobulin, tetanus toxoid and tetanus toxoid in incomplete Freund's adjuvant.

Claim 14

Antibodies raised in an animal host to a synthetic polypeptide containing about 6 to about 10 amino acid residues having an amino acid sequence that immunologically corresponds substantially to an amino acid sequence of an antigenic determinant of interleukin-2, said antibodies having the capacity to immunoreact with said antigenic determinant of interleukin-2.

Claim 15

Antibodies raised in an animal host to a synthetic polypeptide containing about 6 to about 10 amino acid residues having an amino acid sequence that immunologically corresponds substantially to an amino acid sequence of an antigenic determinant of interleukin-2, said antibodies having the capacity to immunoreact with said antigenic determinant of interleukin-2.

Claim 16

Antibodies according to claim 12 wherein said synthetic polypeptide includes a sequence of amino acid residues, taken from left to right and in the direction from amino-terminus to carboxy-terminus, represented by the formula and selected from the group consisting of: ThrAsnSerAlaProThrSerSerSerThrLysLysThrGlnLeu, LeuGluGluGluLeuLysProLeuGluGluValLeuAsnLeu, ValIleValLeuGluLeuLysGlySerGluThrThrPheMetCys;and AsnArgTrpIleThrPheCysGlnSerIleIleSerThrLeuThr.

Claim 17

A diagnostic system for assaying for the presence of an antigenic determinant of interleukin-2 comprising in biochemically active form antibodies raised in an animal host to a synthetic polypeptide containing about 6 to about 40 amino acid residues, having an amino acid sequence that immunologically corresponds substantially to an amino acid sequence of an antigenic determinant of interleukin-2, said antibodies immunoreacting with an admixed sample to be assayed to form an immunoreactant whose presence is signalled by an indicating means.

Claim 18

A diagnostic system for assaying for the presence of an antigenic determinant of interleukin-2 comprising in biochemically active form antibodies raised in an animal host to a synthetic polypeptide containing about 6 to about 40 amino acid residues, having an amino acid sequence that immunologically corresponds substantially to an amino acid sequence of an antigenic determinant of interleukin-2, said antibodies immunoreacting with an admixed sample to be assayed to form an immunoreactant whose presence is signalled by an indicating means.

Claim 19

The diagnostic system according to claim 14 wherein said synthetic polypeptides includes the sequence of amino acid residues, taken from left to right and in the direction from amino-terminus to carboxy-terminus, represented by the formula and selected from the group consisting of: ThrAsnSerAlaProThrSerSerSerThrLysLysThrGlnLeu, LeuGluGluGluLeuLysProLeuGluGluValLeuAsnLeu, ValIleValLeuGluLeuLysGlySerGluThrThrPheMetCys;and AsnArgTrpIleThrPheCysGlnSerIleIleSerThrLeuThr.

Claim 20

The diagnostic system according to claim 14 further including an indicating means comprising enzyme-linked second antibodies, said second antibodies being raised to antibodies of the same class and from the same species as the first named antibodies, and signalling said immunoreaction by binding to said first named antibodies present in said immunoreactant, said signal being indicated by the reaction of said linked enzyme with an added substrate.

Claim 21

The diagnostic system according to claim 14 wherein said indicating means comprises a radioactive element bonded to said antibodies and said immunoreaction causes precipitation of said immunoreactant containing said radioactive element.

Claim 22

A diagnostic system for assaying for the presence of an antigenic determinant of a interleukin-2 in a body component comprising in separate containers (a) a first reagent that contains in biologically active form a synthetic polypeptide containing a sequence of about six to about forty amino acid residues that immunologically corresponds substantially to an amino acid residue sequence of said antigenic determinant of interleukin-2, said polypeptide, when linked to a carrier as a conjugate and introduced in an effective amount as a vaccine into a host animal, being capable of inducing production of antibodies in the host that immunoreact with said antigenic determinant of interleukin-2; and (b) a second reagent that contains in biochemically active form an anti-polypeptide antibody that immunoreacts with said synthetic polypeptide; along with a means for indicating the presence of an immunoreaction between said first and second reagents; said first and second reagents, when admixed in predetermined amounts in the presence of a predetermined amount of body component to be assayed, providing an amount of immunoreaction signalled by said indicating means, the amount of said immunoreaction being different from a known immunoreaction amount when interleukin-2 including said antigenic determinant is not present in said body component.

Claim 23

A diagnostic system for assaying for the presence of an antigenic determinant of a interleukin-2 in a body component comprising in separate containers (a) a first reagent that contains in biologically active form a synthetic polypeptide containing a sequence of about six to about forty amino acid residues that immunologically corresponds substantially to an amino acid residue sequence of said antigenic determinant of interleukin-2, said polypeptide, when linked to a carrier as a conjugate and introduced in an effective amount as a vaccine into a host animal, being capable of inducing production of antibodies in the host that immunoreact with said antigenic determinant of interleukin-2; and (b) a second reagent that contains in biochemically active form an anti-polypeptide antibody that immunoreacts with said synthetic polypeptide; along with a means for indicating the presence of an immunoreaction between said first and second reagents; said first and second reagents, when admixed in predetermined amounts in the presence of a predetermined amount of body component to be assayed, providing an amount of immunoreaction signalled by said indicating means, the amount of said immunoreaction being different from a known immunoreaction amount when interleukin-2 including said antigenic determinant is not present in said body component.

Claim 24

. A method of forming an anti-polypeptide antibody comprising the steps of: (a) administering to a host a synthetic polypeptide in an amount sufficient to induce the production of antibodies to an antigenic determinant of interleukin-2, said synthetic polypeptide having an amino acid residue sequence that substantially corresponds to the amino acid residue sequence of an antigenic determinant of interleukin-2; and (b) recovering the anti-polypeptide antibodies so produced.

Claim 25

. A method of forming an anti-polypeptide antibody comprising the steps of: (a) administering to a host a synthetic polypeptide in an amount sufficient to induce the production of antibodies to an antigenic determinant of interleukin-2, said synthetic polypeptide having an amino acid residue sequence that substantially corresponds to the amino acid residue sequence of an antigenic determinant of interleukin-2; and (b) recovering the anti-polypeptide antibodies so produced.

Claim 26

A method for assaying for the presence of an antigenic determinant of interleukin-2 in a sample comprising: (a) providing an anti-polypeptide antibody to a synthetic polypeptide of claim 1; (b) admixing a predetermined amount of said anti-polypeptide antibody with a predetermined amount of sample to be assayed for the presence of the antigenic determinant to which the anti-polypeptide antibody binds; (c) maintaining that admixture for a period of time sufficient for said anti-polypeptide antibody to bind to the antigenic determinant present in the admixed sample; and (d) determining the amount of binding between said anti-polypeptide antibody and said antigenic determinant of interleukin-2.

Claim 27

An affinity sorbant comprising an inert, solid support having linked thereto in biochemically active form antibodies raised in an animal host to a synthetic polypeptide containing about 6 to about 40 amino acid residues, having an amino acid sequence that immunologically corresponds substantially to an amino acid sequence of an antigenic determinant of interleukin-2, said affinity sorbant forming a reversible complex when admixed with an aqueous composition containing a interleukin-2.

Claim 28

An affinity sorbant comprising an inert, solid support having linked thereto in biochemically active form antibodies raised in an animal host to a synthetic polypeptide containing about 6 to about 40 amino acid residues, having an amino acid sequence that immunologically corresponds substantially to an amino acid sequence of an antigenic determinant of interleukin-2, said affinity sorbant forming a reversible complex when admixed with an aqueous composition containing a interleukin-2.