Patent US4770185A successfully refreshed from Google Patents.
EP0144084A2
Expired / Lapsed
EP
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Reagent for immunoassay and analytical method using the same
Assignee: Toshiba Corp
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Filing: 1984-11-30
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Granted: —
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Expires: 2004-11-30
Abstract
Abstract A reagent for an immunoassay comprises liposomes; a hydrophilic antigen, antibody or at least a part of the antibody immobilized on the liposomes by a cross-linking method or an active lipid method; and a hydrophilic marker material enclosed in the liposomes, and a method for an immunoassay comprises the steps of: mixing the above reagent with a sample including an antigen or antibody and a complement; and measuring the marker material released out of the liposomes in order to quantitatively determine the antigen or antibody in the sample. The reagent of this invention could allow an accurate determination of the antigen or antibody in a homogeneous system with a high detection sensitivity in a short time.
Inventors
- 👤 Yoshio Ishimori
- 👤 Masato Notsuki
- 👤 Masao Koyama
- 👤 Tatsuji Yasuda
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 10:32
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/543; G01N33/54353; G01N33/58; G01N33/585; G01N33/586
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
42 words
A reagent for an immunoassay which comprises liposomes; a hydrophilic antigen, antibody or at least a part of the antibody immobilized on said liposomes by a cross-linking method or an active lipid method; and a hydrophilic marker material enclosed in the liposomes.
Claim 2
Dependent Claim
42 words
A reagent for an immunoassay which comprises liposomes; a hydrophilic antigen, antibody or at least a part of the antibody immobilized on said liposomes by a cross-linking method or an active lipid method; and a hydrophilic marker material enclosed in the liposomes.
Claim 3
Dependent Claim
33 words
The reagent for an immunoassay according to Claims 1, wherein a cross-linking agent used in said cross-linking agent method is selected from the group consisting of N-succinimidyl-3-(2-pyridyldithio)propionate; N-succinimidyl-4-(p-maleimidophenyl)butylate; N-succinimidyl-4-(p-maleimidophenyl)acetate; N-succinimidyl-4-(p-maleimidophenyl)propionate; N-(y-maleimidobutyryloxy)succinimide; and N-(c-maleimidocaproyloxy)succinimide.
Claim 4
Dependent Claim
28 words
The reagent for an immunoassay according to Claim 1, wherein an activator for a lipid used in said active lipid method is bromonitrile, bromoacetylhydroxysuccin- imide or cyanuric chloride.
Claim 5
Dependent Claim
29 words
The reagent for an immunoassay according to Claim 1, wherein said marker material is a fluorescent compound, liminous compound, absorptive compound, saccharide, ionic compound, enzyme, coenzyme or radical compound.
Claim 6
Dependent Claim
50 words
The reagent for an immunoassay according to Claim 1, wherein said hydrophilic antigen or antibody is selected from the group consisting of tumor markers of AFP, BFP, CEA or POA; immunoglobulins of IgA, IgE, IgG or IgM; hormones of insulin, T3 or T4 ; and drugs of phenitoin or digoxin.
Claim 7
Dependent Claim
21 words
The reagent for an immunoassay according to Claim 1, wherein said liposomes are composed of a phospholipid and/or glycopilid and cholesterol.
Claim 8
Dependent Claim
26 words
The reagent for an immunoassay according to Claim 6, wherein the molar ratio of said cholesterol to said phospholipid and/or glycolipid is between 0.1 and 5.0.
Claim 9
Dependent Claim
24 words
The reagent for an immunoassay according to Claim 7, wherein the molar ratio of said cholesterol to said phospholipid and/or glycolipid is 1. -
Claim 10
Dependent Claim
48 words
The reagent for an immunoassay according to Claim 6, wherein with regard to said phospholipid and/or glycolipid constituting said liposomes, an amount of said phospholipid and/or glycolipid which has reacted with a cross-linking agent in said cross-linking method is within the range of 0.01 to 30 mol %.
Claim 11
Dependent Claim
50 words
The reagent for an immunoassay according to Claim 6,_ wherein a concentration of the hydrophilic antigen, antibody or at least a part of said antibody to be immobilized is within the range of 0.01 to 20 mg/ml with respect to 0.5 mM of said liposomes in terms of the lipid.
Claim 12
Independent Claim
85 words
A method for an immunoassay which comprises the steps of: mixing, with a sample including an antigen or antibody and a complement, a reagent for the immunoassay comprising liposomes; a hydrophilic antigen, antibody or at least a part of the antibody immobilized on said liposomes by a cross-linking method or an active lipid method; and a hydrophilic marker material enclosed in said liposomes; and measuring said marker material released out of said liposomes in order to quantitatively determine said antigen or antibody in said sample.
Claim 13
Independent Claim
85 words
A method for an immunoassay which comprises the steps of: mixing, with a sample including an antigen or antibody and a complement, a reagent for the immunoassay comprising liposomes; a hydrophilic antigen, antibody or at least a part of the antibody immobilized on said liposomes by a cross-linking method or an active lipid method; and a hydrophilic marker material enclosed in said liposomes; and measuring said marker material released out of said liposomes in order to quantitatively determine said antigen or antibody in said sample.
Claim 14
Dependent Claim
23 words
The method for an immunoassay according to Claim 11, wherein a complement value of said complement is 0.1 to 10 CH 50 .
References Cited (Backward Citations)
No citation records indexed.
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