EP0141373A2
Expired / Lapsed
EP
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Delivery system for meristematic tissue
Assignee: Monsanto Co
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Filing: 1984-10-24
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Granted: —
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Expires: 2004-10-24
Abstract
Abstract Novel methods and compositions are provided for the delivery of plant meristematic tissue to an environment for growth and development. Isolated meristematic tissue from various sources are combined with beneficial adjuvants in an encapsulating gel matrix which provides a system for the simultaneous delivery of meristematic tissue and adjuvants which can replace true botantic seed.
Inventors
- 👤 Keith M. Redenbaugh
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 10:15
CPC Classifications
A; A01; A01H; A01H4/00; A01H4/005; A01H4/006
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
48 words
A method for delivering meristematic tissue to an environment for growth and development comprising: isolating meristematic tissue with the potential to produce an entire plant body; encapsulating said tissue together with at least one adjuvant capable of affecting the meristematic tissue, the resulting plant body or the environment.
Claim 2
Independent Claim
48 words
A method for delivering meristematic tissue to an environment for growth and development comprising: isolating meristematic tissue with the potential to produce an entire plant body; encapsulating said tissue together with at least one adjuvant capable of affecting the meristematic tissue, the resulting plant body or the environment.
Claim 3
Dependent Claim
28 words
A method as recited in Claim 1 wherein said meristematic tissue is selected from the group consisting of somatic-derived meristematic tissue, botanic seed, zygotic tissue and germline tissue.
Claim 4
Independent Claim
36 words
A method for delivering meristematic tissue to an environment for growth and development comprising encapsulating botanic seed together with at least one adjuvant capable of affecting the botanic seed, the resulting plant body or the environment.
Claim 5
Independent Claim
36 words
A method for delivering meristematic tissue to an environment for growth and development comprising encapsulating botanic seed together with at least one adjuvant capable of affecting the botanic seed, the resulting plant body or the environment.
Claim 6
Dependent Claim
36 words
A method as recited in Claim 1 or 3 wherein said adjuvant is selected from the group consisting of pesticides, herbicides, insecticides, fungicides, fumigants, repellents, rodenticides, fertilizers, nutrients, sugars, carbohydrates, ATP, microorganisms, growth regulators and hormones.
Claim 7
Dependent Claim
16 words
A method as recited in Claim 1 wherein the meristematic tissue is isolated by somatic embryogenesis.
Claim 8
Dependent Claim
38 words
A method as recited in Claim 1 or 3 wherein the meristematic tissue is encapsulated in at least one gel selected from the group consisting of alginate, carrageenan, locust bean gum and gel agents identified in Table 1.
Claim 9
Dependent Claim
64 words
A method as recited in Claim 1 or 3 wherein the meristematic tissue is encapsulated together with at least one adjuvant selected from the group consisting of bensulide, EPTC, metribuzin, pebulate, prometryn, pronamide, chlorpropham, alachlor, diazinon, superphosphate, calcium phosphate, potassium phosphate, potassium nitrate, calcium nitrate, ammonium nitrate, starch, sucrose, Rhizobia meliloti, Rhizobia lequminosarum, Azospirillum, Bacillus thuringiensis, Eschericia coli and adjuvants identified in Table 2.
Claim 10
Dependent Claim
12 words
Meristematic tissue encapsulated in a hydrogel together with at least one adjuvant.
Claim 11
Dependent Claim
12 words
Meristematic tissue encapsulated in a hydrogel together with at least one adjuvant.
Claim 12
Dependent Claim
73 words
A meristematic tissue delivery system for delivering totipotent meristematic tissue to an environment for growth and development which comprises: isolated meristematic tissue having the potential to produce an entire plant body; at least one adjuvant in a concentration capable of affecting the meristematic tissue, the plant body or the environment; and a gel matrix for encapsulating said meristematic tissue and said adjuvant, whereby said tissue and adjuvant are delivered simultaneously to the environment.
Claim 13
Dependent Claim
73 words
A meristematic tissue delivery system for delivering totipotent meristematic tissue to an environment for growth and development which comprises: isolated meristematic tissue having the potential to produce an entire plant body; at least one adjuvant in a concentration capable of affecting the meristematic tissue, the plant body or the environment; and a gel matrix for encapsulating said meristematic tissue and said adjuvant, whereby said tissue and adjuvant are delivered simultaneously to the environment.
Claim 14
Dependent Claim
28 words
A system as recited in Claim 9 wherein said meristematic tissue is selected from the group consisting of somatic-derived meristematic tissue, botanic seed, zygotic tissue and germline tissue.
Claim 15
Dependent Claim
64 words
A meristematic tissue delivery system for delivering totipotent meristematic tissue to an environment for growth and development which comprises: botanic seed; at least one adjuvant in a concentration capable of affecting the botanic seed, the resulting plant body or the environment; and a hydrogel matrix for encapsulating said botanic seed and the adjuvant, whereby said seed and adjuvant are delivered simultaneously to the environment.
Claim 16
Dependent Claim
64 words
A meristematic tissue delivery system for delivering totipotent meristematic tissue to an environment for growth and development which comprises: botanic seed; at least one adjuvant in a concentration capable of affecting the botanic seed, the resulting plant body or the environment; and a hydrogel matrix for encapsulating said botanic seed and the adjuvant, whereby said seed and adjuvant are delivered simultaneously to the environment.
Claim 17
Dependent Claim
42 words
A system as recited in Claim 9 or 11, the gel matrix further comprising distinct regions of the gel matrix containing each adjuvant and the meristematic tissue, whereby the adjuvant, tissue and the environment are allowed to interact in a progressive manner.
Claim 18
Dependent Claim
27 words
A system as recited in Claim 12 wherein each distinct region of the gel matrix comprises a gel agent distinct from the gel agents in adjacent regions.
Claim 19
Dependent Claim
16 words
A system as recited in Claim 10 wherein the meristematic tissue is isolated by somatic embryogenesis.
Claim 20
Dependent Claim
38 words
A system as recited in Claim 9 or 11, wherein the meristematic tissue is encapsulated in at least one gel selected from the group consisting of alginate, carrageenan, locust bean gum and gel agents identified in Table 1.
Claim 21
Dependent Claim
64 words
A system as recited in Claim 9 or 11, wherein the meristematic tissue is encapsulated together with at least one adjuvant selected from the group consisting of bensulide, EPTC, metribuzin, pebulate, protetryn, pronamide, chlorpropham, alachlor, diazinon, superphosphate, calcium phosphate, potassium phosphate, potassium phosphate, calcium nitrate, ammonium nitrate, starch, sucrose, Rhizobia meliloti, Rhizobia leguminosarum, Asospirillum, Bacillus thuringiensis, Eschericia coli and adjuvants identified in Table 2.
References Cited (Backward Citations)
No citation records indexed.
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