EP0107509A2
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EP
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Protein A material and preparation thereof
Assignee: Repligen Corp
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Filing: 1983-10-26
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Granted: —
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Expires: 2003-10-26
Abstract
Abstract The gene coding for a protein A-like material has been cloned and expressed. The cloning of this gene with its nucleotide sequence characterization, enables quantities of a protein A-like material nucleotide sequence for cloning to be obtained in various host-vector systems. Protein A is a valuable component of a variety of diagnostic test systems. The protein A-like material, and subfragments thereof, have the protein A properties of binding to IgG at the Fc region and activation of polyclonal antibody synthesis.
Inventors
- 👤 Donald A. Colbert
- 👤 Algis Anilionis
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 12:25
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/195; C07K14/305; C07K14/31; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
44 words
A microorganism comprising a recombinant plasmid containing a nucleotide sequence coding for the amino acid sequence of protein A-like material, and equivalent nucleotide sequences coding for molecules with substantially the same protein A-like biological activity, characterised in that said nucleotide sequence is as follows:
Claim 2
Dependent Claim
44 words
A microorganism comprising a recombinant plasmid containing a nucleotide sequence coding for the amino acid sequence of protein A-like material, and equivalent nucleotide sequences coding for molecules with substantially the same protein A-like biological activity, characterised in that said nucleotide sequence is as follows:
Claim 3
Dependent Claim
43 words
A microorganism comprising a recombinant plasmid containing a nucleotide sequence coding for the amino acid sequence of a protein A-like material, as defined in claim 1, and subfragments of the nucleotide sequence coding for molecules with substantially the same protein A-like biological activity.
Claim 4
Dependent Claim
55 words
A microorganism comprising a recombinant plasmid containing a nucleotide sequence coding for the amino acid sequence of a protein A-like material, and equivalent nucleotide sequences coding for molecules with substantially the same protein A-like biological activity, said nucleotide sequence being as defined in claim 1 except for the absence of the last 209 nucleotide bases.
Claim 5
Dependent Claim
16 words
A DNA transfer vector comprising a nucleotide sequence as defined in claim 1 or claim 3.
Claim 6
Dependent Claim
15 words
A DNA transfer vector according to claim 4 transferred to and replicated in a microorganism.
Claim 7
Dependent Claim
22 words
Plasmid pAc37 or pAc37-6 comprising the entire genome of pBR322 and the nucleotide sequence defined in claim 1 or claim 3 respectively.
Claim 8
Dependent Claim
9 words
E. coli deposit number NRRL B-15127, or NRRL B-15131
Claim 9
Dependent Claim
9 words
E. coli deposit number NRRL B-15127, or NRRL B-15131
Claim 10
Dependent Claim
48 words
A microorganism comprising a recombinant plasmid containing a nucleotide sequence coding for the amino acid sequence of domain D of a protein A-like material, and equivalent nucleotide sequences coding for molecules with substantially the same domain D biological activity, characterised in that said nucleotide sequence is as follows:
Claim 11
Dependent Claim
48 words
A microorganism comprising a recombinant plasmid containing a nucleotide sequence coding for the amino acid sequence of domain D of a protein A-like material, and equivalent nucleotide sequences coding for molecules with substantially the same domain D biological activity, characterised in that said nucleotide sequence is as follows:
Claim 12
Dependent Claim
48 words
A microorganism comprising a recombinant plasmid containing a nucleotide sequence coding for the amino acid sequence of domain A of a protein A-like material, and equivalent nucleotide sequences coding for molecules with substantially the same domain A biological activity, characterised in that said nucleotide sequence is as follows:
Claim 13
Dependent Claim
48 words
A microorganism comprising a recombinant plasmid containing a nucleotide sequence coding for the amino acid sequence of domain A of a protein A-like material, and equivalent nucleotide sequences coding for molecules with substantially the same domain A biological activity, characterised in that said nucleotide sequence is as follows:
Claim 14
Dependent Claim
48 words
A microorganism comprising a recombinant plasmid containing a nucleotide sequence coding for the amino acid sequence of domain B of a protein A-like material, and equivalent nucleotide sequences coding for molecules with substantially the same domain B biological activity, characterised in that said nucleotide sequence is as follows:
Claim 15
Dependent Claim
48 words
A microorganism comprising a recombinant plasmid containing a nucleotide sequence coding for the amino acid sequence of domain B of a protein A-like material, and equivalent nucleotide sequences coding for molecules with substantially the same domain B biological activity, characterised in that said nucleotide sequence is as follows:
Claim 18
Dependent Claim
48 words
A microorganism comprising a recombinant plasmid containing a nucleotide sequence coding for the amino acid sequence of domain C of a protein A-like material, and equivalent nucleotide sequences coding for molecules with substantially the same domain C biological activity, characterised in that said nucleotide sequence is as follows:
Claim 19
Dependent Claim
48 words
A microorganism comprising a recombinant plasmid containing a nucleotide sequence coding for the amino acid sequence of domain C of a protein A-like material, and equivalent nucleotide sequences coding for molecules with substantially the same domain C biological activity, characterised in that said nucleotide sequence is as follows:
Claim 20
Dependent Claim
48 words
A microorganism comprising a recombinant plasmid containing a nucleotide sequence coding for the amino acid sequence of domain E of a protein A-like material, and equivalent nucleotide sequences coding for molecules with substantially the same domain E biological activity, characterised in that said nucleotide sequence is as follows:
Claim 21
Dependent Claim
48 words
A microorganism comprising a recombinant plasmid containing a nucleotide sequence coding for the amino acid sequence of domain E of a protein A-like material, and equivalent nucleotide sequences coding for molecules with substantially the same domain E biological activity, characterised in that said nucleotide sequence is as follows:
Claim 22
Dependent Claim
48 words
A microorganism comprising a recombinant plasmid containing a nucleotide sequence coding for the amino acid sequence of the carboxy terminal domain of a protein A-like material, and equivalent nucleotide sequences coding for molecules with substantially the same biological activity, characterised in that said nucleotide sequence is as follows:
Claim 23
Dependent Claim
48 words
A microorganism comprising a recombinant plasmid containing a nucleotide sequence coding for the amino acid sequence of the carboxy terminal domain of a protein A-like material, and equivalent nucleotide sequences coding for molecules with substantially the same biological activity, characterised in that said nucleotide sequence is as follows:
Claim 24
Dependent Claim
41 words
A microorganism comprising a recombinant plasmid containing a mixture of nucleotide sequences of domains E, D, A, B, and C coding for amino acid sequences having protein A-like biological activity, and equivalent nucleotide sequences coding for substantially the same biological activity.
Claim 25
Dependent Claim
41 words
A microorganism comprising a recombinant plasmid containing a mixture of nucleotide sequences of domains E, D, A, B, and C coding for amino acid sequences having protein A-like biological activity, and equivalent nucleotide sequences coding for substantially the same biological activity.
Claim 26
Dependent Claim
25 words
An essentially pure nucleotide sequence coding for the amino acid sequence of a protein A-like material, said nucleotide sequence being as defined in claim 1.
Claim 27
Dependent Claim
26 words
An essentially pure nucleotide sequence coding for the amino acid sequence of domain E, or D, or A, or B, or C, or a mixture thereof.
Claim 28
Dependent Claim
26 words
An essentially pure nucleotide sequence coding for the amino acid sequence of domain E, or D, or A, or B, or C, or a mixture thereof.
Claim 29
Dependent Claim
47 words
A process for preparing a protein A-like material, as defined in any one of claims 1, 3, 8, 9, 10, 11, 12 and 13 which comprises expression of cloned DNA, coding for said protein A-like material, in a suitable microbial host, and recovering said protein A-like material.
Claim 30
Dependent Claim
14 words
Protein A-like material (or subfragments thereof) prepared by a process according to claim 17.
Claim 31
Dependent Claim
22 words
Protein A-like material (or subfragments thereof) as defined in any one of claims 1, 3, 8, 9, 10, 11, 12 and 13.
Claim 32
Dependent Claim
19 words
Protein A-like material (or subfragments thereof) according to claim 18 or claim 19 for use as an anticancer agent.
Claim 33
Dependent Claim
25 words
An in vitro method of diagnosis using Protein A-like material (or subfragments thereof) according to claim 18 or claim 19 as a binder for IgG.
Claim 34
Dependent Claim
21 words
An IgG binding assay using as the binder Protein A-like material (or subfragments thereof) according to claim 18 or claim 19.
Claim 35
Dependent Claim
23 words
Protein A-like material (or subfragments thereof) according to claim 18 or claim 19 for use as an IgG binding agent in vivo diagnosis.
References Cited (Backward Citations)
No citation records indexed.
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