Patent US3410300A successfully refreshed from Google Patents.
EP0078556A1
Expired / Lapsed
EP
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Process for cell disruption
Assignee: Shell Internationale Research Maatschappij BV
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Filing: 1982-10-08
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Granted: —
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Expires: 2002-10-08
Abstract
Abstract Process for disrupting cells by contacting an aqueous, cell-containing medium with a protease enzyme, wherein the enzymic contact is preceded by contact with an ionic surfactant; the polysaccharide solutions thereby produced; and a process for displacing a fluid through a well and/or a permeable subsurface formation communicating with the well by injecting into the well an optionally diluted, aqueous solution of such a polysaccharide.
Inventors
- 👤 John David Downs
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 03:30
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/04; C12N; C12N1/00; C12N1/06; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/874
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
30 words
Process for disrupting cells by contacting an aqueous, cell-containing medium with a protease enzyme, characterised in that the enzymic contact is preceded by contact with an anionic or non-ionic surfactant.
Claim 2
Dependent Claim
30 words
Process for disrupting cells by contacting an aqueous, cell-containing medium with a protease enzyme, characterised in that the enzymic contact is preceded by contact with an anionic or non-ionic surfactant.
Claim 3
Dependent Claim
26 words
Process as claimed in claim 1, characterised in that the cell-containing medium is the broth obtained by fermentation of a nutrient medium with a polysaccharide-producing microorganism.
Claim 4
Dependent Claim
17 words
Process as claimed in claim 2, characterised in that the microorganism is a slime-forming species of Pseudomonas.
Claim 5
Dependent Claim
15 words
Process as claimed in claim 3, characterised in that the microorganism is Pseudomonas NCIB 11592.
Claim 6
Dependent Claim
19 words
Process as claimed in any one of the preceding claims, characterised in that the surfactant is an anionic surfactant.
Claim 7
Dependent Claim
36 words
Process as claimed in claim 5, characterised in that the surfactant is an alkali metal alkyl sulphate, alkylbenzene sulphonate, sulphated alkanol ethoxylate or sulphated alkanol, in which the alkyl chain contains at least 8 carbon atoms.
Claim 8
Dependent Claim
25 words
Process as claimed in any one of the preceding claims, characterised in that the medium is contacted with the surfactant at a pH below 6.0.
Claim 9
Dependent Claim
19 words
Process as claimed in any one of the preceding claims, characterised in that the enzyme is an alkaline protease.
Claim 10
Dependent Claim
60 words
Process as claimed in any one of claims 2-8, characterised in that a substantially cell-free polysaccharide solution suitable for use in enhanced oil recovery is obtained by: (a) cultivation of a polysaccharide-producing microorganism in a nutrient medium; (b) contacting the resultant fermentation broth firstly with an ionic surfactant and subsequently with a protease enzyme; and (c) filtering the resulting solution.
Claim 11
Dependent Claim
17 words
Process as claimed in claim 9 characterised in that the substantially cell-free solution is concentrated by ultrafiltration.
Claim 12
Dependent Claim
20 words
A polysaccharide solution suitable for enhanced oil recovery when produced by a process as claimed in claim 9 or 10.
Claim 13
Dependent Claim
36 words
A process for displacing a fluid through a well and/or a permeable subsurface formation communicating with the well, by injecting into the well an optionally diluted, aqueous solution as claimed in claim 9, 10 or 11.
References Cited (Backward Citations)
CH430949A
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US3855197A
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US3598181A
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FR2264077A1
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FR2273067A1
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