EP0061250A2
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EP
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Improved process for recovering products produced by host organisms
Assignee: Biogen NV
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Filing: 1982-03-08
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Granted: —
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Expires: 2002-03-08
Abstract
Abstract An improved process for recovering a product from a host organism that produces such product. The process is charaterized by the step of exposing a host organism that produces a product to at least one composition which causes lysis or at least permeabiiization of the host organism and release of the desired product into the culture medium in recoverable form.
Inventors
- 👤 Charles Weissman
- 👤 Catherine Schein
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 07:49
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/06; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/555; C12P; C12P1/00
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
45 words
A process for recovering a product from a ' host organism that produces said product characterized by the step of exposing the host organism to at least one composition which causes lysis on at least permeablization of the host organism'and release of the desired product.
Claim 2
Dependent Claim
45 words
A process for recovering a product from a ' host organism that produces said product characterized by the step of exposing the host organism to at least one composition which causes lysis on at least permeablization of the host organism'and release of the desired product.
Claim 3
Dependent Claim
39 words
The process of claim 1 characterized in that the host organism is selected from the group consisting of strains of E.coli, Pseudomonas, bacillus subtilis, bacillus stearothermophilus, other bacilli, yeasts, other fungi, animal or plant cells and other host organisms.
Claim 4
Dependent Claim
18 words
The process of claim 1 or 2 characterized in that the host organism is a strain of E.coli.
Claim 5
Dependent Claim
36 words
The process of any one of claims 1 to 3 characterized in that the host organism is a host organism that has been transformed with a vector comprising a DNA sequence that codes for said product.
Claim 6
Dependent Claim
61 words
The process of claim 4 characterized in that said DNA sequence is selected from the group consisting of DNA sequences that code for proteins, DNA sequences that code for polypeptides, DNA sequences that code for single or multiple amino acids, DNA sequences that code for enzymes, DNA sequences that code for industrial chemicals and DNA sequences that code for food stuffs.
Claim 7
Dependent Claim
98 words
The process of claim 4 or 5 characterized in that said DNA sequence is selected from the group consisting of DNA sequences that code for polypeptides displaying an immunological or biological activity of leukocyte interferon, DNA sequences that code for polypeptides displaying an immunological or biological activity of fibroblast interferon, DNA sequences that code for polypeptides having the antigenicity of FMDV viral antigens, DNA sequences that code for polypeptides having the antigenicity of hepatitis B viral antigens, and DNA sequences that code for other polypeptides and proteins displaying an immunological or biological activity of animal or human products.
Claim 8
Dependent Claim
27 words
The process of any one of claims 4 to 6 characterized that said DNA sequence codes for polypeptide displaying an immunological or biological activity of leukocyte interferon.
Claim 9
Dependent Claim
33 words
The process of any one of claims 1 to 7 characterized in that said composition is selected from the group consisting of detergents, enzymes, penicillin, cycloserine, bacitracin, zancomycin, ristocetin, polymyxin and their derivatives.
Claim 10
Dependent Claim
21 words
The process of claim 8 characterized in that said composition is selected from the group consisting of penicillin and derivatives thereof.
Claim 11
Dependent Claim
28 words
The process of claim 9 wherein said penicillin .is added to the culture of about 10 cells/ml in an amount from about 15 µg/l to about 75 mg/1.
References Cited (Backward Citations)
No citation records indexed.
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