EP0060129A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Vaccines, a method for making vaccines and a method for stimulating antibody production
Assignee: Novartis Vaccines and Diagnostics Inc
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Filing: 1982-03-08
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Granted: —
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Expires: 2002-03-08
Abstract
Abstract A vaccine is described for prevention of gastroenteric disease caused in a mammalian species by a pathogenic microorganism and which avoids, in its manufacture, the use of pathogens (which must be attenuated or killed) and the attendant risks, costs and complexity. The present vaccine comprises a nonpathogenic microorganism strain containing stable replicative plasmids, each having one or more genes non-indigenous to the plasmid. The non-indigenous genes are either genes for an adhesin necessay for adherence of the pathogenic microorganism in the mammalian species or are genes for toxoids of toxins causative of the disease. Both types of genes may also be included in the same plasmid. The invention includes the method of manufacture of such vaccines and their use in stimulating the production of antibodies.
Inventors
- 👤 Walter Southwick Dallas
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 15:18
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/02; A61K39/025; A61K39/0258; C; C07; C07K; C07K14/00; C07K14/195; C07K14/24; C07K14/245; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/67; C12N15/68; A61K2039/51; A61K2039/52; A61K2039/523; Y; Y02; Y02A; Y02A50/00; Y02A50/30
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
64 words
A vaccine against gastro-enteric disease caused in a mammalian species by a pathogenic microorganism and comprising a non-pathogenic microorganism strain containing stable replicative plasmids each having a gene or genes non-indigenous to the plasmid and selected from genes for an adhesin necessary for the adherence of the pathogenic microorganism in the mammalian species and/or genes for toxoids of a toxin causative of the disease.
Claim 2
Dependent Claim
64 words
A vaccine against gastro-enteric disease caused in a mammalian species by a pathogenic microorganism and comprising a non-pathogenic microorganism strain containing stable replicative plasmids each having a gene or genes non-indigenous to the plasmid and selected from genes for an adhesin necessary for the adherence of the pathogenic microorganism in the mammalian species and/or genes for toxoids of a toxin causative of the disease.
Claim 3
Dependent Claim
18 words
A vaccine as claimed in Claim 1, wherein said plasmids lack any gene or genes for antibiotic resistance.
Claim 4
Dependent Claim
18 words
A vaccine as claimed in claim 1 or claim 2, wherein said plasmids contain the DNA partition fragment.
Claim 5
Dependent Claim
33 words
A vaccine as claimed in claim 1, wherein both the pathogenic microorganism and the non- pathogenic microorganism are of the species E. coli and the adhesin is selected from K88, K99 and 987P.
Claim 6
Dependent Claim
28 words
A vaccine as claimed in claim 1, wherein both the pathogenic microorganism and the non- pathogenic microorganism are of the species E. coli and the toxoid is LT-B.
Claim 7
Dependent Claim
17 words
A vaccine as claimed in claim 1, wherein the disease is cholera and the toxoid is LT-B.
Claim 8
Independent Claim
81 words
A method for stimulating in a member of a mammalian species the production of antibodies against pathogens associated with gastroenteric disease caused by a pathogenic microorganism, which method comprises challenging said member with a non-pathogenic microorganism strain containing stable replicative plasmids each having a gene or genes non-indigenous to the plasmid and selected from genes for an adhesin necessary for the adherence of the pathogenic microorganism in the mammalian species and/or genes for toxoids of a toxin causative of the disease.
Claim 9
Independent Claim
81 words
A method for stimulating in a member of a mammalian species the production of antibodies against pathogens associated with gastroenteric disease caused by a pathogenic microorganism, which method comprises challenging said member with a non-pathogenic microorganism strain containing stable replicative plasmids each having a gene or genes non-indigenous to the plasmid and selected from genes for an adhesin necessary for the adherence of the pathogenic microorganism in the mammalian species and/or genes for toxoids of a toxin causative of the disease.
Claim 10
Dependent Claim
21 words
A method as claimed in claim 7 further defined by the feature (s) of any one of claims 2 to 6.
Claim 11
Independent Claim
70 words
A method for making a vaccine against gastroenteric disease caused in a mammalian species by a pathogenic microorganism, which method comprises introducing into a non-pathogenic microorganism strain stable replicative plasmids each having a gene or genes non-indigenous to the plasmid and selected from genes for an adhesin necessary for the adherence of the pathogenic microorganism in the mammalian species and/or genes for toxoids of a toxin causative of the disease.
Claim 12
Independent Claim
70 words
A method for making a vaccine against gastroenteric disease caused in a mammalian species by a pathogenic microorganism, which method comprises introducing into a non-pathogenic microorganism strain stable replicative plasmids each having a gene or genes non-indigenous to the plasmid and selected from genes for an adhesin necessary for the adherence of the pathogenic microorganism in the mammalian species and/or genes for toxoids of a toxin causative of the disease.
Claim 13
Dependent Claim
20 words
A method as claimed in claim 9 further defined by the feature(s) of any one of claims 2 to 6.
References Cited (Backward Citations)
No citation records indexed.
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