EP0054249A1
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EP
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Immunoparticles and process for preparing the same
Assignee: Toray Industries Inc
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Filing: 1981-12-08
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Granted: —
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Expires: 2001-12-08
Abstract
Abstract Immunochemicals having an amino group are immobilized by covalent bonding on fine particles having an average diameter of 0.03 to 10 µm, the fine particles comprising a polymer having the repeating unit of glycidyl acrylate or glycidyl methacrylate and the fine particles not having on the surface thereof a hydrophobic component other than the above unit, whereby there are obtained immunoparticles effective as a diagnostic reagent for immunological tests for detecting or measuring a component in human or animal body fluids or for labeling cells.
Inventors
- 👤 Shuntaro Hosaka
- 👤 Yasuo Murao
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-10-23 08:05
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; A; A61; A61K; A61K39/00; A61K39/385; A61K39/395; A61K39/44; C; C08; C08F; C08F220/00; C08F220/02; C08F220/10; C08F220/26; C08F220/32; C08F220/325; A61K2039/60; A61K2039/6093
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
77 words
Immunoparticles comprising fine particles having an average diameter in the range of from 0.03 to 10 pm and an immunochemical having an amino group and immobilized on said fine particles by covalent bonding, said fine particles comprising a polymer having the repeating unit of at least one member selected from the group consisting of glycidyl acrylate and glycidyl methacrylate, and said fine particles not substantially having on the surface thereof a hydrophobic component other than said unit.
Claim 2
Dependent Claim
77 words
Immunoparticles comprising fine particles having an average diameter in the range of from 0.03 to 10 pm and an immunochemical having an amino group and immobilized on said fine particles by covalent bonding, said fine particles comprising a polymer having the repeating unit of at least one member selected from the group consisting of glycidyl acrylate and glycidyl methacrylate, and said fine particles not substantially having on the surface thereof a hydrophobic component other than said unit.
Claim 3
Dependent Claim
27 words
Immunoparticles according to claim 1, wherein said polymer is a copolymer of glycidyl acrylate and/or glycidyl methacrylate and a water-soluble monomer having an unsaturated carbon-carbon double bond.
Claim 4
Dependent Claim
42 words
Immunoparticles according to claim 2, wherein the ratio of the sum of glycidyl acrylate and glycidyl methacrylate to the sum of said water-soluble monomer is in the range of from 100 : 0 to 5 : 95 in terms of molar ratio.
Claim 5
Dependent Claim
53 words
Immunoparticles according to claim 2, wherein said water-soluble monomer is at least one monomer selected from the group consisting of 2-hydroxyethyl acrylate, 2-hydroxyethyl methacrylate, 2-hydroxypropyl acrylate, 2-hydroxypropyl methacrylate, the acrylic or methacrylic acid ester of polyethyleneglycol monoalkylether having a degree of polymerization ranging from 2 to 25, acrylamide, methacrylamide, N-vinylpyrrolidone and glycerol methacrylate.
Claim 6
Dependent Claim
82 words
Immunoparticles according to claim 1, wherein said immunochemical is Treponema pallidum antigen, hepatitis B surface antigen, anti-hepatitis B surface antigen antibody, rubella viral antigen, toxoplasma antigen, streptolysin 0, anti-streptolysin 0 antibody, mycoplasma antigen, human chorionic gonadtropin, anti-human chorionic gonadtropin antibody, aggregated human IgG, rheumatoid factor, nuclear protein, DNA, anti-DNA antibody, C-reactive protein, anti-C-reactive protein antibody, anti-estrogen antibody, a-fetoprotein, anti-α-fetoprotein antibody, carcinoembryonic antigen, anti-carcinoembryonic antigen antibody, Clq, anti-Clq antibody, C3, anti-C3 antibody, anti-C3b antibody, anti-C3bi antibody, C4, anti-C4 antibody, protein-A, conglutinin, or immunoconglutinin.
Claim 7
Dependent Claim
74 words
A process for preparing immunoparticles, comprising polymerizing at least one monomer selected from the group consisting of glycidyl acrylate and glycidyl methacrylate in a medium in which said monomer is soluble but in which the polymer produced is not soluble, thereby precipitating a fine particulate polymer having an average particle diameter in the range of from 0.03 to 10 µm, and then reacting said fine particulate polymer with an immunochemical having an amino group.
Claim 8
Dependent Claim
74 words
A process for preparing immunoparticles, comprising polymerizing at least one monomer selected from the group consisting of glycidyl acrylate and glycidyl methacrylate in a medium in which said monomer is soluble but in which the polymer produced is not soluble, thereby precipitating a fine particulate polymer having an average particle diameter in the range of from 0.03 to 10 µm, and then reacting said fine particulate polymer with an immunochemical having an amino group.
Claim 9
Dependent Claim
46 words
A process according to claim 6, wherein said medium is ethyl acetate, n-propyl acetate, isopropyl acetate, butyl acetates, ethyl propionate, n-propyl propionate, isopropyl propionate, butyl propionates, methyl ethyl ketone, methyl n-propyl ketone, methyl isopropyl ketone, methyl butyl ketones, benzene, toluene, o-xylene, m-xylene, p-xylene, or carbon tetrachloride.
Claim 10
Dependent Claim
24 words
A process according to claim 6, wherein said monomer is polymerized in the presence of a water-soluble monomer having an unsaturated carbon-carbon double bond.
Claim 11
Dependent Claim
32 words
A process according to claim 6, wherein said monomer is polymerized in the presence of a water-soluble monomer having an unsaturated carbon-carbon double bond and a cross-linking agent and a polymerization initiator.
Claim 12
Dependent Claim
33 words
A process according to claim 6, wherein either simul traneouely with or after the reaction of said fine particulate polymer with said immunochemical, said fine particulate polymer is reacted with a hydrophilic protein.
Claim 13
Dependent Claim
27 words
A process according to claim 6, wherein before the reaction of said fine particulate polymer with said immunochemical, said fine particulate polymer is treated with tannic acid.
References Cited (Backward Citations)
GB137184A
Cited
GB1424967A
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GB2009184A
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DE2839737A1
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GB2041940A
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EP0038960A2
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