CA1104110A
Expired / Lapsed
CA
⏱️ 0.0 years remaining
Enzyme-immobilization carriers and preparation thereof
Assignee: Sumitomo Chemical Co Ltd
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Filing: 1978-04-27
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Granted: 1981-06-30
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Expires: 1998-04-27
Abstract
Abstract ABSTRACT OF THE DISCLOSURE Enzyme-immobilization carriers are provided herein. They comprise a macroporous amphoteric ion-exchange resin having a cation-exchange capacity due to carboxymethyl groups of 0.5 meq/g-dry resin or more, an anion-exchange capacity of 1 meq/g-dry resin or more, and a specific area of 1 m2/g-dry resin or more, the total volume of macropores having a diameter of 100 .ANG. to 2000 .ANG. being 0.1 cc/g-dry resin or more. A novel process for producing such carriers is also provided. Such resins are superior as carriers for enzyme immobilization, and provide immobilized enzymes having great stability.
Inventors
- 👤 Tsuneyuki Nagase
- 👤 Shigeyasu Nabeshima
- 👤 Hideo Hirohara
Bibliographic Details
Application Number
—
Jurisdiction
CA
Data Source
google_patents
Last Synchronized
2026-10-23 09:52
CPC Classifications
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Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
64 words
An enzyme-immobilization carrier which comprises a macro-porous amphoteric ion-exchange resin having a cation-exchange capacity due to carboxymethyl groups of 0.5 meq/g-dry resin or more, an anion-ex-change capacity of 1 meq/g-dry resin or more, and a specific surface area of 1 m2/g-dry resin or more, the total volume of macropores having a dia-meter of 100 .ANG. to 2000 .ANG. being 0.1 cc/g-dry resin or more.
Claim 2
Dependent Claim
64 words
An enzyme-immobilization carrier which comprises a macro-porous amphoteric ion-exchange resin having a cation-exchange capacity due to carboxymethyl groups of 0.5 meq/g-dry resin or more, an anion-ex-change capacity of 1 meq/g-dry resin or more, and a specific surface area of 1 m2/g-dry resin or more, the total volume of macropores having a dia-meter of 100 .ANG. to 2000 .ANG. being 0.1 cc/g-dry resin or more.
Claim 3
Dependent Claim
21 words
An enzyme-immobilization carrier according to Claim 1, wherein said specific surface area of the resin is 5 m2/g-dry resin or more.
Claim 4
Dependent Claim
25 words
An enzyme-immobilization carrier according to Claim 1, wherein the mean pore diameter of the pores in said resin is from 150 .ANG. to 1000 .ANG..
Claim 5
Dependent Claim
28 words
An enzyme-immobilization carrier accord;ng to Claim 1, wherein said total volume of macropores having a diameter of 100 .ANG. to 2000 .ANG. is 0.2 cc/g-dry resin or more.
Claim 6
Dependent Claim
115 words
A process for producing an enzyme-immobilization carrier which comprises reacting a compound of the formula, wherein X is a halogen atom and Y is a hydrogen atom or an alkali metal, with a macroporous anion-exchange resin having a functional group capable of reacting with said compound, an anion-exchange capacity of 1 meq/g-dry resin or more, and a specific surface area of 1 m2/g-dry resin or more, the total volume of macropores having a diameter of 100 .ANG. to 2000 .ANG. being 0.1 cc/g-dry resin or more, in the presence of an alkaline compound, whereby 0.5 meq/g-dry resin or more of carboxymethyl groups is introduced into said macroporous anion-exchange resin to obtain an am-photeric ion-exchange resin.
Claim 7
Dependent Claim
115 words
A process for producing an enzyme-immobilization carrier which comprises reacting a compound of the formula, wherein X is a halogen atom and Y is a hydrogen atom or an alkali metal, with a macroporous anion-exchange resin having a functional group capable of reacting with said compound, an anion-exchange capacity of 1 meq/g-dry resin or more, and a specific surface area of 1 m2/g-dry resin or more, the total volume of macropores having a diameter of 100 .ANG. to 2000 .ANG. being 0.1 cc/g-dry resin or more, in the presence of an alkaline compound, whereby 0.5 meq/g-dry resin or more of carboxymethyl groups is introduced into said macroporous anion-exchange resin to obtain an am-photeric ion-exchange resin.
Claim 8
Dependent Claim
15 words
A process according to Claim 5, wherein said compound is chloroacetic acid or sodium chloroacetate.
Claim 9
Dependent Claim
27 words
A process according to Claim 5, wherein said compound is used in an amount of 1/2 to 10 parts based on 1 part of the dry resin.
Claim 10
Dependent Claim
27 words
A process according to Claim 7, wherein said compound is used in an amount of 2/3 to 3 parts based on 1 part of the dry resin.
Claim 11
Dependent Claim
22 words
A process according to Claim 5, wherein said alkaline com-pound is an alkali metal hydroxide, alkaline earth metal hydroxide or organic amine.
Claim 12
Dependent Claim
13 words
A process according to Claim 9, wherein said alkaline com-pound is sodium hydroxide.
Claim 13
Dependent Claim
26 words
A process according to Claim 5, wherein said alkaline com-pound is used in an amount of 1/3 to 2 times by mole based on said com-pound.
Claim 14
Dependent Claim
21 words
A process according to Claim 5, wherein said anion-exchange resin has a hydroxyl, primary amino, secondary amino, imino or sulfhydryl group.
Claim 15
Dependent Claim
20 words
A process according to Claim 5, wherein said specific sur-face area of the resin is 5 m2/g-dry resin or more.
Claim 16
Dependent Claim
24 words
A process according to Claim 5, wherein the mean pore dia-meter of the pores in said resin is from 150 .ANG. to 1000 .ANG..
Claim 17
Dependent Claim
27 words
A process according to Claim 5, wherein said total volume of macropores having a diameter of 100 .ANG. to 2000 .ANG. is 0.2 cc/g-dry resin or more.
Claim 18
Dependent Claim
22 words
A process for immobilizing an enzyme on the enzyme-immobili-zation carrier which comprises immobilizing an enzyme on the enzyme-immobilization carrier of Claim 1.
Claim 19
Dependent Claim
17 words
A process as in Claim 16, wherein the enzyme is carried on the resin by adsorption method.
Claim 20
Dependent Claim
18 words
A process as in Claim 16, wherein the enzyme is carried on the resin by covalent attachment method.
References Cited (Backward Citations)
No citation records indexed.
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